Improving Bioprocess Conditions for the Production of Prodigiosin Using a Marine Strain

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2024 Apr 26

PMID 38667759

Authors

Ricardo F S Pereira

Carla C C R de Carvalho

Affiliations

Soon will be listed here.

Abstract

The enormous potential attributed to prodigiosin regarding its applicability as a natural pigment and pharmaceutical agent justifies the development of sound bioprocesses for its production. Using a strain isolated from a shallow-water hydrothermal vent, optimization of the growth medium composition was carried out. After medium development, the bacterium temperature, light and oxygen needs were studied, as was growth inhibition by product concentration. The implemented changes led to a 13-fold increase in prodigiosin production in a shake flask, reaching 19.7 mg/L. The conditions allowing the highest bacterial cell growth and prodigiosin production were also tested with another marine strain: isolated from a tide rock pool was able to produce 15.8 mg/L of prodigiosin. The bioprocess with was scaled up from 0.1 L shake flasks to 2 L bioreactors using the maintenance of the oxygen mass transfer coefficient () as the scale-up criterion. The implemented parameters in the bioreactor led to an 8-fold increase in product per biomass yield and to a final concentration of 293.1 mg/L of prodigiosin in 24 h.

Citing Articles

Lipid- and Multivariate-Based Analyses to Determine Cell Response to pH Variations and Buffer Composition.

Pereira R, de Carvalho C Mar Biotechnol (NY). 2025; 27(1):48.

PMID: 39954186 PMC: 11829930. DOI: 10.1007/s10126-025-10421-4.

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