Antennal Transcriptome Evaluation and Analysis for Odorant-Binding Proteins, Chemosensory Proteins, and Suitable Reference Genes in the Leaf Beetle Pest Weise (Coleoptera: Chrysomelidae)

Overview

Journal Insects

Specialty Biology

Date 2024 Apr 26

PMID 38667381

Authors

Bo-Xin Xi

Xiao-Ning Cui

Su-Qin Shang

Guang-Wei Li

Youssef Dewer

Chang-Ning Li

Gui-Xin Hu

Yan Wang

Affiliations

Soon will be listed here.

Abstract

Weise is one of the dominant pests feeding on spp., a pioneer plant used for windbreaking and sand fixation purposes, and poses a threat to local livestock and ecosystems. To clarify the key olfactory genes of and provide a theoretical basis for attractant and repellent development, the optimal reference genes under two different conditions (tissue and sex) were identified, and the bioinformatics and characterization of the tissue expression profiles of two categories of soluble olfactory proteins (OBPs and CSPs) were investigated. The results showed that the best reference genes were and for comparison among tissues, and and for comparison between sexes. Strong expressions of , , , , , , and were found in antennae, the most important olfactory organ for . These findings not only provide a basis for further in-depth research on the olfactory molecular mechanisms of host-specialized pests but also provide a theoretical basis for the future development of new chemical attractants or repellents using volatiles to control .

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