Epidemiologic Survey of Crimean-Congo Hemorrhagic Fever Virus in Suids, Spain

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2024 Apr 26

PMID 38666621

Authors

Mario Frias

Kerstin Fischer

Sabrina Castro-Scholten

Caroline Bost

David Cano-Terriza

Maria Angeles Risalde

Pelayo Acevedo

Saul Jimenez-Ruiz

Balal Sadeghi

Martin H Groschup

Javier Caballero-Gomez

Ignacio Garcia-Bocanegra

Affiliations

Soon will be listed here.

Abstract

We conducted a cross-sectional study in wild boar and extensively managed Iberian pig populations in a hotspot area of Crimean-Congo hemorrhagic fever virus (CCHFV) in Spain. We tested for antibodies against CCHFV by using 2 ELISAs in parallel. We assessed the presence of CCHFV RNA by means of reverse transcription quantitative PCR protocol, which detects all genotypes. A total of 113 (21.8%) of 518 suids sampled showed antibodies against CCHFV by ELISA. By species, 106 (39.7%) of 267 wild boars and 7 (2.8%) of 251 Iberian pigs analyzed were seropositive. Of the 231 Iberian pigs and 231 wild boars analyzed, none tested positive for CCHFV RNA. These findings indicate high CCHFV exposure in wild boar populations in endemic areas and confirm the susceptibility of extensively reared pigs to CCHFV, even though they may only play a limited role in the enzootic cycle.

Citing Articles

Crimean-Congo Hemorrhagic Fever Virus: An Emerging Threat in Europe with a Focus on Epidemiology in Spain.

Eslava M, Carlos S, Reina G Pathogens. 2024; 13(9).

PMID: 39338961 PMC: 11434923. DOI: 10.3390/pathogens13090770.

References

Estrada-Pena A, Palomar A, Santibanez P, Sanchez N, Habela M, Portillo A . Crimean-Congo hemorrhagic fever virus in ticks, Southwestern Europe, 2010. Emerg Infect Dis. 2012; 18(1):179-80. PMC: 3310114. DOI: 10.3201/eid1801.111040. View

Valcarcel F, Gonzalez J, Gonzalez M, Sanchez M, Tercero J, Elhachimi L . Comparative Ecology of and Koch, 1844 (Acarina: Ixodidae). Insects. 2020; 11(5). PMC: 7290797. DOI: 10.3390/insects11050303. View

Phonera M, Simuunza M, Kainga H, Ndebe J, Chembensofu M, Chatanga E . Seroprevalence and Risk Factors of Crimean-Congo Hemorrhagic Fever in Cattle of Smallholder Farmers in Central Malawi. Pathogens. 2021; 10(12). PMC: 8709441. DOI: 10.3390/pathogens10121613. View

Castillo-Contreras R, Magen L, Birtles R, Varela-Castro L, Hall J, Conejero C . Ticks on wild boar in the metropolitan area of Barcelona (Spain) are infected with spotted fever group rickettsiae. Transbound Emerg Dis. 2021; 69(4):e82-e95. DOI: 10.1111/tbed.14268. View

Sanchez-Seco M, Sierra M, Estrada-Pena A, Valcarcel F, Molina R, de Arellano E . Widespread Detection of Multiple Strains of Crimean-Congo Hemorrhagic Fever Virus in Ticks, Spain. Emerg Infect Dis. 2022; 28(2):394-402. PMC: 8798670. DOI: 10.3201/eid2802.211308. View

Baz-Flores S, Herraiz C, Peralbo-Moreno A, Barral M, Arnal M, Balseiro A . Mapping the risk of exposure to Crimean-Congo haemorrhagic fever virus in the Iberian Peninsula using Eurasian wild boar (Sus scrofa) as a model. Ticks Tick Borne Dis. 2023; 15(1):102281. DOI: 10.1016/j.ttbdis.2023.102281. View

Ruiz-Fons F, Fernandez-de-Mera I, Acevedo P, Hofle U, Vicente J, de la Fuente J . Ixodid ticks parasitizing Iberian red deer (Cervus elaphus hispanicus) and European wild boar (Sus scrofa) from Spain: geographical and temporal distribution. Vet Parasitol. 2006; 140(1-2):133-42. DOI: 10.1016/j.vetpar.2006.03.033. View

Negredo A, Sanchez-Arroyo R, Diez-Fuertes F, de Ory F, Budino M, Vazquez A . Fatal Case of Crimean-Congo Hemorrhagic Fever Caused by Reassortant Virus, Spain, 2018. Emerg Infect Dis. 2021; 27(4):1211-1215. PMC: 8007309. DOI: 10.3201/eid2704.203462. View

Nurettin C, Engin B, Sukru T, Munir A, Zati V, Aykut O . The Seroprevalence of Crimean-Congo Hemorrhagic Fever in Wild and Domestic Animals: An Epidemiological Update for Domestic Animals and First Seroevidence in Wild Animals from Turkiye. Vet Sci. 2022; 9(9). PMC: 9501697. DOI: 10.3390/vetsci9090462. View

10.

Bost C, Castro-Scholten S, Sadeghi B, Cano-Terriza D, Frias M, Jimenez-Ruiz S . Approaching the complexity of Crimean-Congo hemorrhagic fever virus serology: A study in swine. J Virol Methods. 2024; 326:114915. DOI: 10.1016/j.jviromet.2024.114915. View

11.

Balinandi S, von Bromssen C, Tumusiime A, Kyondo J, Kwon H, Monteil V . Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda. J Virol Methods. 2021; 290:114075. DOI: 10.1016/j.jviromet.2021.114075. View

12.

Sas M, Comtet L, Donnet F, Mertens M, Vatansever Z, Tordo N . A novel double-antigen sandwich ELISA for the species-independent detection of Crimean-Congo hemorrhagic fever virus-specific antibodies. Antiviral Res. 2018; 151:24-26. DOI: 10.1016/j.antiviral.2018.01.006. View

13.

Diaz-Cao J, Adaszek L, Dziegiel B, Paniagua J, Caballero-Gomez J, Winiarczyk S . Prevalence of selected tick-borne pathogens in wild ungulates and ticks in southern Spain. Transbound Emerg Dis. 2021; 69(3):1084-1094. DOI: 10.1111/tbed.14065. View

14.

Carrera-Faja L, Cardells J, Pailler-Garcia L, Lizana V, Alfaro-Deval G, Espunyes J . Evidence of Prolonged Crimean-Congo Hemorrhagic Fever Virus Endemicity by Retrospective Serosurvey, Eastern Spain. Emerg Infect Dis. 2022; 28(5):1031-1034. PMC: 9045418. DOI: 10.3201/eid2805.212335. View

15.

Goletic T, Satrovic L, Softic A, Omeragic J, Goletic S, Klaric Soldo D . Serologic and molecular evidence for circulation of Crimean-Congo hemorrhagic fever virus in ticks and cattle in Bosnia and Herzegovina. Ticks Tick Borne Dis. 2022; 13(5):102004. DOI: 10.1016/j.ttbdis.2022.102004. View

16.

Cuadrado-Matias R, Baz-Flores S, Peralbo-Moreno A, Herrero-Garcia G, Risalde M, Barroso P . Determinants of Crimean-Congo haemorrhagic fever virus exposure dynamics in Mediterranean environments. Transbound Emerg Dis. 2022; 69(6):3571-3581. PMC: 10092370. DOI: 10.1111/tbed.14720. View

17.

Darwish M, IMAM I, Omar F, HOOGSTRAAL H . Results of a preliminary seroepidemiological survey for Crimean-Congo hemorrhagic fever virus in Egypt. Acta Virol. 1978; 22(1):77. View

18.

Monsalve Arteaga L, Munoz Bellido J, Negredo A, Garcia Criado J, Vieira Lista M, Sanchez Serrano J . New circulation of genotype V of Crimean-Congo haemorrhagic fever virus in humans from Spain. PLoS Negl Trop Dis. 2021; 15(2):e0009197. PMC: 7943016. DOI: 10.1371/journal.pntd.0009197. View

19.

Moraga-Fernandez A, Ruiz-Fons F, Habela M, Royo-Hernandez L, Calero-Bernal R, Gortazar C . Detection of new Crimean-Congo haemorrhagic fever virus genotypes in ticks feeding on deer and wild boar, Spain. Transbound Emerg Dis. 2020; 68(3):993-1000. DOI: 10.1111/tbed.13756. View

20.

Hawman D, Feldmann H . Recent advances in understanding Crimean-Congo hemorrhagic fever virus. F1000Res. 2018; 7. PMC: 6206615. DOI: 10.12688/f1000research.16189.1. View