Illuminating the Role of Chaperones in Spliceosome Biogenesis and Recycling

Overview

Journal Nat Struct Mol Biol

Specialties Cell Biology
Molecular Biology

Date 2024 Apr 25

PMID 38664567

Authors

Suyang Zhang

Affiliations

Soon will be listed here.

References

Kastner B, Will C, Stark H, Luhrmann R . Structural Insights into Nuclear pre-mRNA Splicing in Higher Eukaryotes. Cold Spring Harb Perspect Biol. 2019; 11(11). PMC: 6824238. DOI: 10.1101/cshperspect.a032417. View

Bach M, Luhrmann R . Protein-RNA interactions in 20S U5 snRNPs. Biochim Biophys Acta. 1991; 1088(1):139-43. DOI: 10.1016/0167-4781(91)90164-h. View

Achsel T, Ahrens K, Brahms H, Teigelkamp S, Luhrmann R . The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein. Mol Cell Biol. 1998; 18(11):6756-66. PMC: 109259. DOI: 10.1128/MCB.18.11.6756. View

Malinova A, Cvackova Z, Mateju D, Horejsi Z, Abeza C, Vandermoere F . Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones. J Cell Biol. 2017; 216(6):1579-1596. PMC: 5461031. DOI: 10.1083/jcb.201701165. View

Klimesova K, Vojackova J, Radivojevic N, Vandermoere F, Bertrand E, Verheggen C . TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation. Nat Commun. 2021; 12(1):3646. PMC: 8206348. DOI: 10.1038/s41467-021-23934-y. View

Laggerbauer B, Liu S, Makarov E, Vornlocher H, Makarova O, Ingelfinger D . The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation. RNA. 2005; 11(5):598-608. PMC: 1370748. DOI: 10.1261/rna.2300805. View

Nielsen T, Liu S, Luhrmann R, Ficner R . Structural basis for the bifunctionality of the U5 snRNP 52K protein (CD2BP2). J Mol Biol. 2007; 369(4):902-8. DOI: 10.1016/j.jmb.2007.03.077. View

Galej W, Oubridge C, Newman A, Nagai K . Crystal structure of Prp8 reveals active site cavity of the spliceosome. Nature. 2013; 493(7434):638-43. PMC: 3672837. DOI: 10.1038/nature11843. View

Weber G, Cristao V, Santos K, Jovin S, Heroven A, Holton N . Structural basis for dual roles of Aar2p in U5 snRNP assembly. Genes Dev. 2013; 27(5):525-40. PMC: 3605466. DOI: 10.1101/gad.213207.113. View

10.

Agafonov D, Kastner B, Dybkov O, Hofele R, Liu W, Urlaub H . Molecular architecture of the human U4/U6.U5 tri-snRNP. Science. 2016; 351(6280):1416-20. DOI: 10.1126/science.aad2085. View

11.

Charenton C, Wilkinson M, Nagai K . Mechanism of 5' splice site transfer for human spliceosome activation. Science. 2019; 364(6438):362-367. PMC: 6525098. DOI: 10.1126/science.aax3289. View

12.

Bergfort A, Hilal T, Kuropka B, Ilik I, Weber G, Aktas T . The intrinsically disordered TSSC4 protein acts as a helicase inhibitor, placeholder and multi-interaction coordinator during snRNP assembly and recycling. Nucleic Acids Res. 2022; 50(5):2938-2958. PMC: 8934646. DOI: 10.1093/nar/gkac087. View

13.

Grainger R, Beggs J . Prp8 protein: at the heart of the spliceosome. RNA. 2005; 11(5):533-57. PMC: 1370742. DOI: 10.1261/rna.2220705. View