Complete Genome Sequence of the Novel Virulent Phage PMBT24 Infecting from the Human Gut

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Apr 24

PMID 38655313

Authors

Sabrina Sprotte

Erik Brinks

Horst Neve

Charles M A P Franz

Affiliations

Soon will be listed here.

Abstract

PMBT24, the first reported virulent bacteriophage infecting the anaerobic human gut bacterium strain MBT-21, was isolated from a municipal sewage sample and its genome was sequenced and analysed. Transmission electron microscopy revealed a phage with an icosahedral head and a long, non-contractile tail. The circularly permutated, 99,962-bp dsDNA genome of the type phage has a mol% G + C content of 32.1 and comprises 173 putative ORFs. Using amino acid sequence-based phylogeny, phage PMBT24 showed similarity to other, hitherto non-published phage genomes in the databases. Our data suggested phage PMBT24 to present the type phage of a novel genus (proposed name Kielvirus) and novel family of phages (proposed name Kielviridae).

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