Phytoalexin Sakuranetin Attenuates Endocytosis and Enhances Resistance to Rice Blast

Overview

Journal Nat Commun

Specialty Biology

Date 2024 Apr 23

PMID 38653755

Authors

Lihui Jiang

Xiaoyan Zhang

Yiting Zhao

Haiyan Zhu

Qijing Fu

Xinqi Lu

Wuying Huang

Xinyue Yang

Xuan Zhou

Lixia Wu

Ao Yang

Xie He

Man Dong

Ziai Peng

Jing Yang

Liwei Guo

Jiancheng Wen

Huichuan Huang

Yong Xie

Shusheng Zhu

Chengyun Li

Xiahong He

Youyong Zhu

Jiri Friml

Yunlong Du

Affiliations

Soon will be listed here.

Abstract

Phytoalexin sakuranetin functions in resistance against rice blast. However, the mechanisms underlying the effects of sakuranetin remains elusive. Here, we report that rice lines expressing resistance (R) genes were found to contain high levels of sakuranetin, which correlates with attenuated endocytic trafficking of plasma membrane (PM) proteins. Exogenous and endogenous sakuranetin attenuates the endocytosis of various PM proteins and the fungal effector PWL2. Moreover, accumulation of the avirulence protein AvrCO39, resulting from uptake into rice cells by Magnaporthe oryzae, was reduced following treatment with sakuranetin. Pharmacological manipulation of clathrin-mediated endocytic (CME) suggests that this pathway is targeted by sakuranetin. Indeed, attenuation of CME by sakuranetin is sufficient to convey resistance against rice blast. Our data reveals a mechanism of rice against M. oryzae by increasing sakuranetin levels and repressing the CME of pathogen effectors, which is distinct from the action of many R genes that mainly function by modulating transcription.

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