Surface Property and Toxicity Effect of Insoluble Particles Given by Protein Corona: Implication for PM Cytotoxicity Assessment

Overview

Journal Eco Environ Health

Date 2024 Apr 19

PMID 38638169

Authors

Sisi Chen

Yexuan Zhang

Hongjuan Chen

Weijuan Zheng

Xin Hu

Li Mao

Xuewen Guo

Hongzhen Lian

Affiliations

Soon will be listed here.

Abstract

toxicological assessment helps explore key fractions of particulate matter (PM) in association with the toxic mechanism. Previous studies mainly discussed the toxicity effects of the water-soluble and organic-soluble fractions of PM. However, the toxicity of insoluble fractions is relatively poorly understood, and the adsorption of proteins is rarely considered. In this work, the formation of protein corona on the surface of insoluble particles during incubation in a culture medium was investigated. It was found that highly abundant proteins in fetal bovine serum were the main components of the protein corona. The adsorbed proteins increased the dispersion stability of insoluble particles. Meanwhile, the leaching concentrations of some metal elements (e.g., Cu, Zn, and Pb) from PM increased in the presence of proteins. The toxicity effects and potential mechanisms of the PM insoluble particle-protein corona complex on macrophage cells RAW264.7 were discussed. The results revealed that the PM insoluble particle-protein corona complex could influence the phagosome pathway in RAW264.7 cells. Thus, it promoted the intracellular reactive oxygen species generation and induced a greater degree of cell differentiation, significantly altering cell morphology. Consequently, this work sheds new light on the combination of insoluble particles and protein corona in terms of PM cytotoxicity assessment.

References

Pi J, Li T, Liu J, Su X, Wang R, Yang F . Detection of lipopolysaccharide induced inflammatory responses in RAW264.7 macrophages using atomic force microscope. Micron. 2014; 65:1-9. DOI: 10.1016/j.micron.2014.03.012. View

Lu D, Luo Q, Chen R, Zhuansun Y, Jiang J, Wang W . Chemical multi-fingerprinting of exogenous ultrafine particles in human serum and pleural effusion. Nat Commun. 2020; 11(1):2567. PMC: 7244483. DOI: 10.1038/s41467-020-16427-x. View

Zhou M, Wang H, Zeng X, Yin P, Zhu J, Chen W . Mortality, morbidity, and risk factors in China and its provinces, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet. 2019; 394(10204):1145-1158. PMC: 6891889. DOI: 10.1016/S0140-6736(19)30427-1. View

Chen S, Wang T, Song W, Tang Z, Cao Z, Chen H . A novel particulate matter sampling and cell exposure strategy based on agar membrane for cytotoxicity study. Chemosphere. 2022; 300:134473. DOI: 10.1016/j.chemosphere.2022.134473. View

Park S, Choi E, Suh K, Kim H, Chin S, Rhee S . Tetrabromobisphenol A Promotes the Osteoclastogenesis of RAW264.7 Cells Induced by Receptor Activator of NF-kappa B Ligand In Vitro. J Korean Med Sci. 2019; 34(41):e267. PMC: 6813422. DOI: 10.3346/jkms.2019.34.e267. View

Liu Z, Zhu Q, Song E, Song Y . Characterization of blood protein adsorption on PM and its implications on cellular uptake and cytotoxicity of PM. J Hazard Mater. 2021; 414:125499. DOI: 10.1016/j.jhazmat.2021.125499. View

Burnett R, Pope 3rd C, Ezzati M, Olives C, Lim S, Mehta S . An integrated risk function for estimating the global burden of disease attributable to ambient fine particulate matter exposure. Environ Health Perspect. 2014; 122(4):397-403. PMC: 3984213. DOI: 10.1289/ehp.1307049. View

Wang Q, Liu Y, Wang H, Jiang P, Qian W, You M . Graphdiyne oxide nanosheets display selective anti-leukemia efficacy against DNMT3A-mutant AML cells. Nat Commun. 2022; 13(1):5657. PMC: 9512932. DOI: 10.1038/s41467-022-33410-w. View

Sarkar J, Wen X, Simanian E, Paine M . V-type ATPase proton pump expression during enamel formation. Matrix Biol. 2015; 52-54:234-245. PMC: 4867139. DOI: 10.1016/j.matbio.2015.11.004. View

10.

Simon J, Wolf T, Klein K, Landfester K, Wurm F, Mailander V . Hydrophilicity Regulates the Stealth Properties of Polyphosphoester-Coated Nanocarriers. Angew Chem Int Ed Engl. 2018; 57(19):5548-5553. DOI: 10.1002/anie.201800272. View

11.

Turchinovich A, Tonevitsky A, Burwinkel B . Extracellular miRNA: A Collision of Two Paradigms. Trends Biochem Sci. 2016; 41(10):883-892. DOI: 10.1016/j.tibs.2016.08.004. View

12.

El-Benna J, Dang P, Gougerot-Pocidalo M, Marie J, Braut-Boucher F . p47phox, the phagocyte NADPH oxidase/NOX2 organizer: structure, phosphorylation and implication in diseases. Exp Mol Med. 2009; 41(4):217-25. PMC: 2679237. DOI: 10.3858/emm.2009.41.4.058. View

13.

Ghazaryan A, Landfester K, Mailander V . Protein deglycosylation can drastically affect the cellular uptake. Nanoscale. 2019; 11(22):10727-10737. DOI: 10.1039/c8nr08305c. View

14.

Wang Z, Zhai Z, Chen C, Tian X, Xing Z, Xing P . Air pollution particles hijack peroxidasin to disrupt immunosurveillance and promote lung cancer. Elife. 2022; 11. PMC: 9054135. DOI: 10.7554/eLife.75345. View

15.

Xu F, Qiu X, Hu X, Shang Y, Pardo M, Fang Y . Effects on IL-1β signaling activation induced by water and organic extracts of fine particulate matter (PM) in vitro. Environ Pollut. 2018; 237:592-600. DOI: 10.1016/j.envpol.2018.02.086. View

16.

Gali N, Li G, Ning Z, Brimblecombe P . Diurnal trends in redox characteristics of water-soluble and -insoluble PM components. Environ Pollut. 2019; 254(Pt A):112841. DOI: 10.1016/j.envpol.2019.07.009. View

17.

Roper C, Delgado L, Barrett D, Massey Simonich S, Tanguay R . PM Filter Extraction Methods: Implications for Chemical and Toxicological Analyses. Environ Sci Technol. 2018; 53(1):434-442. PMC: 6652177. DOI: 10.1021/acs.est.8b04308. View

18.

Wang G, Kawamura K, Lee S, Ho K, Cao J . Molecular, seasonal, and spatial distributions of organic aerosols from fourteen Chinese cities. Environ Sci Technol. 2006; 40(15):4619-25. DOI: 10.1021/es060291x. View

19.

Duan G, Kang S, Tian X, Garate J, Zhao L, Ge C . Protein corona mitigates the cytotoxicity of graphene oxide by reducing its physical interaction with cell membrane. Nanoscale. 2015; 7(37):15214-24. DOI: 10.1039/c5nr01839k. View

20.

Hamra G, Guha N, Cohen A, Laden F, Raaschou-Nielsen O, Samet J . Outdoor particulate matter exposure and lung cancer: a systematic review and meta-analysis. Environ Health Perspect. 2014; 122(9):906-11. PMC: 4154221. DOI: 10.1289/ehp/1408092. View