Milk Microbiota of Holstein Friesian Cattle Reared in Lahore: Association with Mastitis

Overview

Journal Saudi J Biol Sci

Specialty Biology

Date 2024 Apr 18

PMID 38633360

Authors

Mian Muhammad Salman

Muhammad Nawaz

Tahir Yaqub

Muhammad Hassan Mushtaq

Affiliations

Soon will be listed here.

Abstract

The dairy industry is reshaping itself and becoming commercialized in Pakistan due to the increased demand for milk to overcome the shortage. Exotic breeds such as Holstein Friesian, a high milk producing breed have started being reared more on farms in Pakistan. Along with other issues, mastitis does affects the milk production of this breed. The objective of this study was to evaluate the milk composition in terms of bacterial communities in Holstein Friesian reared in Punjab, Pakistan and alteration in microbial composition with healthy and mastitic udder. Milk samples (n = 36) from farms rearing Holstein Friesian were collected. Among these samples, 05 samples from each three groups, HHF(healthy), CHF (clinical mastitis) and SHF (subclinical mastitis), based on their udder health condition, were processed using the 16 S r=RNA gene based technique. Diversity assessment as carried out by alpha diversity indices showed that milk samples from the udder infected with clinical mastitis were the least diverse and those from the healthy udder were more diverse. Beta diversity across samples showed a scattered pattern suggesting overlap amongst bacterial communities across different groups samples as depicted by PCA plots of beta diversity indices. The taxonomic profile revealed that Proteobacteria Firmicutes, Bacteroidota and Actinobacteriota were the major phyla detected across all groups. Proteobacteria dominated the HHF and SHF group while abundance of Firmicutes was higher in CHF group. Differences at other levels including order, genus and species were also recorded. The overall picture concludes that diverse microbiota is associated with different udder health conditions.

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References

Jiang C, Hou X, Gao X, Liu P, Guo X, Hu G . The 16S rDNA high-throughput sequencing correlation analysis of milk and gut microbial communities in mastitis Holstein cows. BMC Microbiol. 2023; 23(1):180. PMC: 10327393. DOI: 10.1186/s12866-023-02925-7. View

Delbes C, Ali-Mandjee L, Montel M . Monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16S rRNA gene-based analyses. Appl Environ Microbiol. 2007; 73(6):1882-91. PMC: 1828836. DOI: 10.1128/AEM.01716-06. View

Kaczorowski L, Powierska-Czarny J, Wolko L, Piotrowska-Cyplik A, Cyplik P, Czarny J . The Influence of Bacteria Causing Subclinical Mastitis on the Structure of the Cow's Milk Microbiome. Molecules. 2022; 27(6). PMC: 8950352. DOI: 10.3390/molecules27061829. View

Taponen S, McGuinness D, Hiitio H, Simojoki H, Zadoks R, Pyorala S . Bovine milk microbiome: a more complex issue than expected. Vet Res. 2019; 50(1):44. PMC: 6555717. DOI: 10.1186/s13567-019-0662-y. View

Metzger S, Hernandez L, Suen G, Ruegg P . Understanding the Milk Microbiota. Vet Clin North Am Food Anim Pract. 2018; 34(3):427-438. DOI: 10.1016/j.cvfa.2018.06.003. View

Steinberg R, Silva E Silva L, de Souza M, Reis R, da Silva P, Lacorte G . Changes in bovine milk bacterial microbiome from healthy and subclinical mastitis affected animals of the Girolando, Gyr, Guzera, and Holstein breeds. Int Microbiol. 2022; 25(4):803-815. DOI: 10.1007/s10123-022-00267-4. View

Lubna , Hussain T, Shami A, Rafiq N, Khan S, Kabir M . Antimicrobial Usage and Detection of Multidrug-Resistant : Methicillin- and Tetracycline-Resistant Strains in Raw Milk of Lactating Dairy Cattle. Antibiotics (Basel). 2023; 12(4). PMC: 10135139. DOI: 10.3390/antibiotics12040673. View

Espeche M, Pellegrino M, Frola I, Larriestra A, Bogni C, Nader-Macias M . Lactic acid bacteria from raw milk as potentially beneficial strains to prevent bovine mastitis. Anaerobe. 2012; 18(1):103-9. DOI: 10.1016/j.anaerobe.2012.01.002. View

Cheng J, Zhou M, Nobrega D, Barkema H, Xu S, Li M . Genetic diversity and molecular epidemiology of outbreaks of Klebsiella pneumoniae mastitis on two large Chinese dairy farms. J Dairy Sci. 2020; 104(1):762-775. DOI: 10.3168/jds.2020-19325. View

10.

Tarrah A, Callegaro S, Pakroo S, Finocchiaro R, Giacomini A, Corich V . New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis. Sci Rep. 2022; 12(1):13498. PMC: 9356063. DOI: 10.1038/s41598-022-17418-2. View

11.

Curone G, Filipe J, Cremonesi P, Trevisi E, Amadori M, Pollera C . What we have lost: Mastitis resistance in Holstein Friesians and in a local cattle breed. Res Vet Sci. 2017; 116:88-98. DOI: 10.1016/j.rvsc.2017.11.020. View

12.

Windsor P, Agerholm J . Inherited diseases of Australian Holstein-Friesian cattle. Aust Vet J. 2009; 87(5):193-9. DOI: 10.1111/j.1751-0813.2009.00422.x. View

13.

Baraniecki C, Aislabie J, Foght J . Characterization of Sphingomonas sp. Ant 17, an aromatic hydrocarbon-degrading bacterium isolated from Antarctic soil. Microb Ecol. 2002; 43(1):44-54. DOI: 10.1007/s00248-001-1019-3. View

14.

Addis M, Tanca A, Uzzau S, Oikonomou G, Bicalho R, Moroni P . The bovine milk microbiota: insights and perspectives from -omics studies. Mol Biosyst. 2016; 12(8):2359-72. DOI: 10.1039/c6mb00217j. View

15.

. Structure, function and diversity of the healthy human microbiome. Nature. 2012; 486(7402):207-14. PMC: 3564958. DOI: 10.1038/nature11234. View

16.

Cremonesi P, Ceccarani C, Curone G, Severgnini M, Pollera C, Bronzo V . Milk microbiome diversity and bacterial group prevalence in a comparison between healthy Holstein Friesian and Rendena cows. PLoS One. 2018; 13(10):e0205054. PMC: 6200206. DOI: 10.1371/journal.pone.0205054. View

17.

Gilbert J, Jansson J, Knight R . The Earth Microbiome project: successes and aspirations. BMC Biol. 2014; 12:69. PMC: 4141107. DOI: 10.1186/s12915-014-0069-1. View

18.

Patel S, Vaidya Y, Patel R, Pandit R, Joshi C, Kunjadiya A . Culture independent assessment of human milk microbial community in lactational mastitis. Sci Rep. 2017; 7(1):7804. PMC: 5552812. DOI: 10.1038/s41598-017-08451-7. View

19.

Igono M, JOHNSON H, Steevens B, Hainen W, Shanklin M . Effect of season on milk temperature, milk growth hormone, prolactin, and somatic cell counts of lactating cattle. Int J Biometeorol. 1988; 32(3):194-200. DOI: 10.1007/BF01045279. View

20.

Ruegg P . The bovine milk microbiome - an evolving science. Domest Anim Endocrinol. 2022; 79:106708. DOI: 10.1016/j.domaniend.2021.106708. View