Sp. Nov., Sp. Nov. and Sp. Nov., Rare Actinobacteria from Miocene Lacustrine Sediment of the Sokolov Coal Basin, Czech Republic

Overview

Journal Int J Syst Evol Microbiol

Specialty Microbiology

Date 2024 Apr 17

PMID 38630118

Authors

Ana Catalina Lara

Lucie Kotrbova

Moritz Keller

Imen Nouioui

Meina Neumann-Schaal

Yvonne Mast

Alica Chronakova

Affiliations

Soon will be listed here.

Abstract

The taxonomic position of three actinobacterial strains, BCCO 10_0061, BCCO 10_0798, and BCCO 10_0856, recovered from bare soil in the Sokolov Coal Basin, Czech Republic, was established using a polyphasic approach. The multilocus sequence analysis based on 100 single-copy genes positioned BCCO 10_0061 in the same cluster as , strain BCCO 10_0798 in the same cluster as , , , and , and strain BCCO 10_0856 clustered together with and . Morphological and chemotaxonomic characteristics of these strains support their assignment to the genus . In all three strains, MK-9(H) accounted for more than 80 % of the isoprenoid quinone. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The whole-cell sugars were rhamnose, ribose, mannose, glucose, and galactose. The major fatty acids (>10 %) were iso-C, anteiso-C, iso-C, and C. The polar lipids were diphosphatidylglycerol, methyl-phosphatidylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol. The genomic DNA G+C content of strains (mol%) was 68.8 for BCCO 10_0061, 69.2 for BCCO 10_0798, and 68.5 for BCCO 10_0856. The combination of digital DNA-DNA hybridization results, average nucleotide identity values and phenotypic characteristics of BCCO 10_0061, BCCO 10_0798, and BCCO 10_0856 distinguishes them from their closely related strains. Bioinformatic analysis of the genome sequences of the strains revealed several biosynthetic gene clusters (BGCs) with identities >50 % to already known clusters, including BGCs for geosmin, coelichelin, ε-poly-l-lysine, and erythromycin-like BGCs. Most of the identified BGCs showed low similarity to known BGCs (<50 %) suggesting their genetic potential for the biosynthesis of novel secondary metabolites. Based on the above results, each strain represents a novel species of the genus , for which we propose the name sp. nov. for BCCO 10_0061 (=DSM 116175), sp. nov. for BCCO 10_0798 (=DSM 116176), and sp. nov. for BCCO 10_0856 (=DSM 116177).

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