Seed Extract Promotes Folliculogenesis and Alleviates the Diminished Ovarian Reserve in the Balb/c Mice Model: An Experimental Study

Overview

Journal Int J Reprod Biomed

Specialty Reproductive Medicine

Date 2024 Apr 17

PMID 38628780

Authors

Sharareh Hekmat

Mohammad Sharifzadeh

Tayebeh Toliyat

Roghayeh Savary Kouzehkonan

Mozhgan Mehri Ardestani

Malihe Tabarrai

Seyede Nargess Sadati Lamardi

Affiliations

Soon will be listed here.

Abstract

Background: seed (UPS) is a Persian traditional medicine prescription that positively affects female infertility.

Objective: This study aimed to evaluate the beneficial effects of UPS on a diminished ovarian reserve (DOR) model induced by cyclophosphamide in Balb/c mice.

Materials And Methods: A single intraperitoneal (75 mg/kg) of cyclophosphamide was administered to establish a DOR model. 25 female Balb/c mice (6-8 wk, 25 2 gr) were randomly divided into 5 groups (n = 5/each), including control (normal saline), model (DOR), DOR+50, DOR+100, and DOR+200 (mg/kg UPS, gavage) groups for 14 days. The levels of follicle-stimulating hormone, luteinizing hormone, estradiol, malondialdehyde, superoxide dismutases, apoptosis, and histopathological alterations were analyzed. Gas chromatography-mass spectrometry analysis was performed to identify the phytochemicals of the UPS.

Results: It was observed that the UPS extract reduced malondialdehyde concentration and apoptosis in the DOR model as well as enhanced superoxide dismutases activity in the ovaries in a dose-dependent manner. Moreover, it exerted a modulatory effect on steroidal hormones such as follicle-stimulating hormone, luteinizing hormone, and estradiol. The histopathological analysis revealed the therapeutic potential of the UPS extract. The main chemical components of UPS were linoleic acid (59.25%), n-hexadecanoic acid (10.36%), and oleic acid (8.29%).

Conclusion: The results indicated that the UPS extract has therapeutic potential in the DOR model. This potential is attributed to the reduction of oxidative stress, modulation of apoptosis, and regulation of steroidal hormones that may be associated with the observed beneficial effects of fatty acids on fertility improvement.

Citing Articles

The biological function of spp. and its application in poultry, fish and livestock.

Gao Y, Yang X, Chen B, Leng H, Zhang J Front Vet Sci. 2024; 11:1430362.

PMID: 39411389 PMC: 11473253. DOI: 10.3389/fvets.2024.1430362.

References

Ozcan P, Ficicioglu C, Yildirim O, Ozkan F, Akkaya H, Aslan I . Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague-Dawley rats. Reprod Biomed Online. 2015; 31(3):404-10. DOI: 10.1016/j.rbmo.2015.06.007. View

Eftekhari Moghadam A, Taheri Moghadam M, Hemadi M, Saki G . Oocyte quality and aging. JBRA Assist Reprod. 2021; 26(1):105-122. PMC: 8769179. DOI: 10.5935/1518-0557.20210026. View

Fayezi S, Leroy J, Novin M, Darabi M . Oleic acid in the modulation of oocyte and preimplantation embryo development. Zygote. 2017; 26(1):1-13. DOI: 10.1017/S0967199417000582. View

Roberts H, Hickey M, Lethaby A . Hormone therapy in postmenopausal women and risk of endometrial hyperplasia: a Cochrane review summary. Maturitas. 2013; 77(1):4-6. DOI: 10.1016/j.maturitas.2013.10.002. View

Jiang M, Wang W, Zhang J, Wang C, Bi Y, Li P . Protective Effects and Possible Mechanisms of Actions of Bushen Cuyun Recipe on Diminished Ovarian Reserve Induced by Cyclophosphamide in Rats. Front Pharmacol. 2020; 11:546. PMC: 7237638. DOI: 10.3389/fphar.2020.00546. View

Vitagliano A, Petre G, Francini-Pesenti F, De Toni L, Di Nisio A, Grande G . Dietary Supplements for Female Infertility: A Critical Review of Their Composition. Nutrients. 2021; 13(10). PMC: 8541636. DOI: 10.3390/nu13103552. View

Wick M . The hematoxylin and eosin stain in anatomic pathology-An often-neglected focus of quality assurance in the laboratory. Semin Diagn Pathol. 2019; 36(5):303-311. DOI: 10.1053/j.semdp.2019.06.003. View

Rieppo L, Janssen L, Rahunen K, Lehenkari P, Finnila M, Saarakkala S . Histochemical quantification of collagen content in articular cartilage. PLoS One. 2019; 14(11):e0224839. PMC: 6837441. DOI: 10.1371/journal.pone.0224839. View

Park S, Walsh L, Berkowitz K . Mechanisms of ovarian aging. Reproduction. 2021; 162(2):R19-R33. PMC: 9354567. DOI: 10.1530/REP-21-0022. View

10.

Lu J, Wang Z, Cao J, Chen Y, Dong Y . A novel and compact review on the role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2018; 16(1):80. PMC: 6102891. DOI: 10.1186/s12958-018-0391-5. View

11.

Skoracka K, Ratajczak A, Rychter A, Dobrowolska A, Krela-Kazmierczak I . Female Fertility and the Nutritional Approach: The Most Essential Aspects. Adv Nutr. 2021; 12(6):2372-2386. PMC: 8634384. DOI: 10.1093/advances/nmab068. View

12.

Huang C, Song K, Ma W, Ding J, Chen Z, Zhang M . Immunomodulatory mechanism of Bushen Huoxue Recipe alleviates cyclophosphamide-induced diminished ovarian reserve in mouse model. J Ethnopharmacol. 2017; 208:44-56. DOI: 10.1016/j.jep.2017.06.022. View

13.

Akbaribazm M, Goodarzi N, Rahimi M . Female infertility and herbal medicine: An overview of the new findings. Food Sci Nutr. 2021; 9(10):5869-5882. PMC: 8498057. DOI: 10.1002/fsn3.2523. View

14.

Qi Q, Zhang X, Yao L, Chen Y, Weng H . Pueratin improves diminished ovarian reserve by inhibiting apoptosis. Exp Ther Med. 2021; 22(6):1423. PMC: 8549093. DOI: 10.3892/etm.2021.10858. View

15.

Song W, Liu M, Zhao Z, Huang C, Xu J, Wang A . SIRT1 Inhibits High Shear Stress-Induced Apoptosis in Rat Cortical Neurons. Cell Mol Bioeng. 2020; 13(6):621-631. PMC: 7704980. DOI: 10.1007/s12195-020-00623-2. View

16.

Amawi K, Alkhatib A . Urtica Pilulifera in Treating Pre-diabetic Rat Model to Control the Blood Glucose, Lipids and Oxidative Stress. Med Arch. 2020; 74(3):168-171. PMC: 7405998. DOI: 10.5455/medarh.2020.74.168-171. View

17.

Jahangirifar M, Taebi M, Nasr-Esfahani M, Heidari-Beni M, Asgari G . Dietary Fatty Acid Intakes and the Outcomes of Assisted Reproductive Technique in Infertile Women. J Reprod Infertil. 2021; 22(3):173-183. PMC: 8607876. DOI: 10.18502/jri.v22i3.6718. View

18.

Yan F, Zhao Q, Li Y, Zheng Z, Kong X, Shu C . The role of oxidative stress in ovarian aging: a review. J Ovarian Res. 2022; 15(1):100. PMC: 9434839. DOI: 10.1186/s13048-022-01032-x. View

19.

Bulbul M, Tekce A, Annac E, Korkmaz O, Onderci M, Korkmaz D . Identification of royal jelly as a potential new drug to protect the ovarian reserve and uterus against cyclophosphamide in rats. Clin Exp Reprod Med. 2023; 50(1):34-43. PMC: 10030208. DOI: 10.5653/cerm.2022.05596. View

20.

Hamzeh M, Hosseinimehr S, Mohammadi H, Yaghubi Beklar S, Dashti A, Talebpour Amiri F . Atorvastatin attenuates the ovarian damage induced by cyclophosphamide in rat: An experimental study. Int J Reprod Biomed. 2018; 16(5):323-334. PMC: 6046206. View