Expression of Hybrid Class I Genes of the Major Histocompatibility Complex in Mouse L Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Sep 1

PMID 3862131

Citations 13

Authors

D S Straus

I Stroynowski

S G Schiffer

L Hood

Affiliations

Soon will be listed here.

Abstract

The class I genes of the major histocompatibility complex of the mouse can be divided into two categories: those encoding the transplantation antigens and those encoding the Qa and Tla antigens. The inbred BALB/c mouse has 28 potential Qa/Tla genes. The sites of tissue expression, developmental regulation, and functions of these genes are virtually unknown. We have used the technique of exon shuffling to construct hybrid genes between each of three Qa region genes (Q5, Q7, and Q8) and two other class I genes (H-2Ld and Q6). The hybrid genes have been transfected into mouse L cells, in which intact transplantation antigen genes generally are expressed and in which intact Qa genes generally are not expressed. Analysis of expression of the hybrid gene constructs indicates that the 5' half of two of the Qa genes (Q5 and Q8) can readily be expressed in the context of a hybrid molecule, whereas the 3' half prevents cell-surface expression. The exon shuffling approach described here will be useful in characterizing Qa/Tla genes and in identifying or producing new reagents to study the Qa/Tla gene products, their tissue distribution, their developmental stages of expression, and, ultimately, their functions.

Citing Articles

Analysis of major histocompatibility complex class I folding: novel insights into intermediate forms.

Simone L, Tuli A, Simone P, Wang X, Solheim J Tissue Antigens. 2012; 79(4):249-62.

PMID: 22329842 PMC: 3310231. DOI: 10.1111/j.1399-0039.2012.01849.x.

Productive association between MHC class I and tapasin requires the tapasin transmembrane/cytosolic region and the tapasin C-terminal Ig-like domain.

Simone L, Georgesen C, Simone P, Wang X, Solheim J Mol Immunol. 2011; 49(4):628-39.

PMID: 22169163 PMC: 3249531. DOI: 10.1016/j.molimm.2011.11.002.

Organization and evolution of D region class I genes in the mouse major histocompatibility complex.

Stephan D, Sun H, Lindahl K, Meyer E, Hammerling G, Hood L J Exp Med. 1986; 163(5):1227-44.

PMID: 3701254 PMC: 2188107. DOI: 10.1084/jem.163.5.1227.

Tissue-specific expression of cell-surface Qa-2 antigen from a transfected Q7b gene of C57BL/10 mice.

Waneck G, Sherman D, Calvin S, Allen H, Flavell R J Exp Med. 1987; 165(5):1358-70.

PMID: 3502706 PMC: 2188306. DOI: 10.1084/jem.165.5.1358.

Qa gene expression: biosynthesis and secretion of Qa-2 molecules in activated T cells.

Soloski M, Vernachio J, Einhorn G, Lattimore A Proc Natl Acad Sci U S A. 1986; 83(9):2949-53.

PMID: 3486423 PMC: 323424. DOI: 10.1073/pnas.83.9.2949.

References

Kincade P, Flaherty L, Lee G, Watanabe T, Michaelson J . Qa antigen expression on functional lymphoid, myeloid, and stem cells in adult mice. J Immunol. 1980; 124(6):2879-85. View

Stroynowski I, Forman J, Goodenow R, Schiffer S, McMillan M, Sharrow S . Expression and T cell recognition of hybrid antigens with amino-terminal domains encoded by Qa-2 region of major histocompatibility complex and carboxyl termini of transplantation antigens. J Exp Med. 1985; 161(5):935-52. PMC: 2187603. DOI: 10.1084/jem.161.5.935. View

Cosman D, Khoury G, Jay G . Three classes of mouse H-2 messenger RNA distinguished by analysis of cDNA clones. Nature. 1982; 295(5844):73-6. DOI: 10.1038/295073a0. View

Steinmetz M, Winoto A, Minard K, Hood L . Clusters of genes encoding mouse transplantation antigens. Cell. 1982; 28(3):489-98. DOI: 10.1016/0092-8674(82)90203-3. View

Lynes M, Tonkonogy S, Flaherty L . Qa-1 and Qa-2 expression on CFU-s. J Immunol. 1982; 129(3):928-30. View

Cosman D, Kress M, Khoury G, Jay G . Tissue-specific expression of an unusual H-2 (class I)-related gene. Proc Natl Acad Sci U S A. 1982; 79(16):4947-51. PMC: 346802. DOI: 10.1073/pnas.79.16.4947. View

Soloski M, McIntyre K, UHR J, Vitetta E . Primary structural analysis of the Tla region gene products, QA-2 and TL. Mol Immunol. 1982; 19(9):1193-7. DOI: 10.1016/0161-5890(82)90330-3. View

Goodenow R, McMillan M, Nicolson M, Sher B, Eakle K, Davidson N . Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer. Nature. 1982; 300(5889):231-7. DOI: 10.1038/300231a0. View

Evans G, Margulies D, Shykind B, Seidman J, Ozato K . Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens. Nature. 1982; 300(5894):755-7. DOI: 10.1038/300755a0. View

10.

Goodenow R, Stroynowski I, McMillan M, Nicolson M, Eakle K, Sher B . Expression of complete transplantation antigens by mammalian cells transformed with truncated class I genes. Nature. 1983; 301(5899):388-94. DOI: 10.1038/301388a0. View

11.

Rebai N, Mercier P, Kristensen T, Devaux C, Malissen B, Mawas C . Murine H-2Dd-reactive monoclonal antibodies recognize shared antigenic determinant(s) on human HLA-B7 or HLA-B27 molecules or both. Immunogenetics. 1983; 17(4):357-70. DOI: 10.1007/BF00372455. View

12.

Winoto A, Steinmetz M, Hood L . Genetic mapping in the major histocompatibility complex by restriction enzyme site polymorphisms: most mouse class I genes map to the Tla complex. Proc Natl Acad Sci U S A. 1983; 80(11):3425-9. PMC: 394056. DOI: 10.1073/pnas.80.11.3425. View

13.

Kress M, Cosman D, Khoury G, Jay G . Secretion of a transplantation-related antigen. Cell. 1983; 34(1):189-96. DOI: 10.1016/0092-8674(83)90149-6. View

14.

Steinmetz M, Hood L . Genes of the major histocompatibility complex in mouse and man. Science. 1983; 222(4625):727-33. DOI: 10.1126/science.6356354. View

15.

Mellor A, Weiss E, Kress M, Jay G, Flavell R . A nonpolymorphic class I gene in the murine major histocompatibility complex. Cell. 1984; 36(1):139-44. DOI: 10.1016/0092-8674(84)90082-5. View

16.

Sharrow S, Flaherty L, Sachs D . Serologic cross-reactivity between Class I MHC molecules and an H-2-linked differentiation antigen as detected by monoclonal antibodies. J Exp Med. 1984; 159(1):21-40. PMC: 2187195. DOI: 10.1084/jem.159.1.21. View

17.

Maloy W, Coligan J, Barra Y, Jay G . Detection of a secreted form of the murine H-2 class I antigen with an antibody against its predicted carboxyl terminus. Proc Natl Acad Sci U S A. 1984; 81(4):1216-20. PMC: 344797. DOI: 10.1073/pnas.81.4.1216. View

18.

Michaelson J, Boyse E, Chorney M, Flaherty L, Fleissner E, Hammerling U . The biochemical genetics of the Qa-Tla region. Transplant Proc. 1983; 15(4):2033-8. View

19.

Allen H, Wraith D, Pala P, Askonas B, Flavell R . Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature. 1984; 309(5965):279-81. DOI: 10.1038/309279a0. View

20.

Arnold B, Burgert H, Hamann U, Hammerling G, Kees U, Kvist S . Cytolytic T cells recognize the two amino-terminal domains of H-2 K antigens in tandem in influenza A infected cells. Cell. 1984; 38(1):79-87. DOI: 10.1016/0092-8674(84)90528-2. View