African Swine Fever Virus PB318L, a Trans-geranylgeranyl-diphosphate Synthase, Negatively Regulates CGAS-STING and IFNAR-JAK-STAT Signaling Pathways

Overview

Journal PLoS Pathog

Specialty Microbiology

Date 2024 Apr 15

PMID 38620034

Authors

Xiaohong Liu

Hefeng Chen

Guangqiang Ye

Hongyang Liu

Chunying Feng

Weiye Chen

Liang Hu

Qiongqiong Zhou

Zhaoxia Zhang

Jiangnan Li

Xianfeng Zhang

Xijun He

Yuntao Guan

Zhengshuang Wu

Dongming Zhao

Zhigao Bu

Changjiang Weng

Li Huang

Affiliations

Soon will be listed here.

Abstract

African swine fever (ASF) is an acute, hemorrhagic, and severe infectious disease caused by the ASF virus (ASFV). ASFV has evolved multiple strategies to escape host antiviral immune responses. Here, we reported that ASFV pB318L, a trans-geranylgeranyl-diphosphate synthase, reduced the expression of type I interferon (IFN-I) and IFN-stimulated genes (ISGs). Mechanically, pB318L not only interacted with STING to reduce the translocation of STING from the endoplasmic reticulum to the Golgi apparatus but also interacted with IFN receptors to reduce the interaction of IFNAR1/TYK2 and IFNAR2/JAK1. Of note, ASFV with interruption of B318L gene (ASFV-intB318L) infected PAMs produces more IFN-I and ISGs than that in PAMs infected with its parental ASFV HLJ/18 at the late stage of infection. Consistently, the pathogenicity of ASFV-intB318L is attenuated in piglets compared with its parental virus. Taken together, our data reveal that B318L gene may partially affect ASFV pathogenicity by reducing the production of IFN-I and ISGs. This study provides a clue to design antiviral agents or live attenuated vaccines to prevent and control ASF.

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