Effects of Lactobacillus-fermented Low-protein Diets on the Growth Performance, Nitrogen Excretion, Fecal Microbiota and Metabolomic Profiles of Finishing Pigs

Overview

Journal Sci Rep

Specialty Science

Date 2024 Apr 14

PMID 38616198

Authors

Hui Liu

Sixin Wang

Meixia Chen

Haifeng Ji

Dongyan Zhang

Affiliations

Soon will be listed here.

Abstract

This study investigated the effects of Lactobacillus-fermented low-protein diet on the growth performance, nitrogen balance, fecal microbiota, and metabolomic profiles of finishing pigs. A total of 90 finishing pigs were assigned to one of three dietary treatments including a normal protein diet (CON) as well as two experimental diets in which a low-protein diet supplemented with 0 (LP) or 1% Lactobacillus-fermented low-protein feed (FLP). In comparison with CON, the LP and FLP significantly increased average daily gain (P = 0.044), significantly decreased feed to gain ratio (P = 0.021), fecal nitrogen (P < 0.01), urine nitrogen (P < 0.01), and total nitrogen (P < 0.01), respectively. The LP group exhibited increased abundances of unclassified_f_Selenomonadaceae, Coprococcus, Faecalibacterium, and Butyricicoccus, while the abundances of Verrucomicrobiae, Verrucomicrobiales, Akkermansiaceae, and Akkermansia were enriched in the FLP group. Low-protein diet-induced metabolic changes were enriched in sesquiterpenoid and triterpenoid biosynthesis and Lactobacillus-fermented low-protein feed-induced metabolic changes were enriched in phenylpropanoid biosynthesis and arginine biosynthesis. Overall, low-protein diet and Lactobacillus-fermented low-protein diet improved the growth performance and reduce nitrogen excretion, possibly via altering the fecal microbiota and metabolites in the finishing pigs. The present study provides novel ideas regarding the application of the low-protein diet and Lactobacillus-fermented low-protein diet in swine production.

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References

Rotz C . Management to reduce nitrogen losses in animal production. J Anim Sci. 2004; 82 E-Suppl:E119-137. DOI: 10.2527/2004.8213_supplE119x. View

Fan P, Liu P, Song P, Chen X, Ma X . Moderate dietary protein restriction alters the composition of gut microbiota and improves ileal barrier function in adult pig model. Sci Rep. 2017; 7:43412. PMC: 5333114. DOI: 10.1038/srep43412. View

Deryabina I, Andrianov V, Muranova L, Bogodvid T, Gainutdinov K . Effects of Thryptophan Hydroxylase Blockade by P-Chlorophenylalanine on Contextual Memory Reconsolidation after Training of Different Intensity. Int J Mol Sci. 2020; 21(6). PMC: 7139692. DOI: 10.3390/ijms21062087. View

Spring S, Premathilake H, Bradway C, Shili C, DeSilva U, Carter S . Effect of very low-protein diets supplemented with branched-chain amino acids on energy balance, plasma metabolomics and fecal microbiome of pigs. Sci Rep. 2020; 10(1):15859. PMC: 7523006. DOI: 10.1038/s41598-020-72816-8. View

Xiang Q, Wu X, Pan Y, Wang L, Cui C, Guo Y . Early-Life Intervention Using Fecal Microbiota Combined with Probiotics Promotes Gut Microbiota Maturation, Regulates Immune System Development, and Alleviates Weaning Stress in Piglets. Int J Mol Sci. 2020; 21(2). PMC: 7014131. DOI: 10.3390/ijms21020503. View

Isaacson R, Kim H . The intestinal microbiome of the pig. Anim Health Res Rev. 2012; 13(1):100-9. DOI: 10.1017/S1466252312000084. View

Bergamaschi M, Tiezzi F, Howard J, Huang Y, Gray K, Schillebeeckx C . Gut microbiome composition differences among breeds impact feed efficiency in swine. Microbiome. 2020; 8(1):110. PMC: 7376719. DOI: 10.1186/s40168-020-00888-9. View

Nie C, He T, Zhang W, Zhang G, Ma X . Branched Chain Amino Acids: Beyond Nutrition Metabolism. Int J Mol Sci. 2018; 19(4). PMC: 5979320. DOI: 10.3390/ijms19040954. View

Luo Z, Li C, Cheng Y, Hang S, Zhu W . Effects of low dietary protein on the metabolites and microbial communities in the caecal digesta of piglets. Arch Anim Nutr. 2015; 69(3):212-26. DOI: 10.1080/1745039X.2015.1034521. View

10.

Zhang S, Chu L, Qiao S, Mao X, Zeng X . Effects of dietary leucine supplementation in low crude protein diets on performance, nitrogen balance, whole-body protein turnover, carcass characteristics and meat quality of finishing pigs. Anim Sci J. 2015; 87(7):911-20. DOI: 10.1111/asj.12520. View

11.

Mu C, Yang Y, Yu K, Yu M, Zhang C, Su Y . Alteration of metabolomic markers of amino-acid metabolism in piglets with in-feed antibiotics. Amino Acids. 2017; 49(4):771-781. DOI: 10.1007/s00726-017-2379-4. View

12.

Liu S, Du M, Tu Y, You W, Chen W, Liu G . Fermented mixed feed alters growth performance, carcass traits, meat quality and muscle fatty acid and amino acid profiles in finishing pigs. Anim Nutr. 2023; 12:87-95. PMC: 9822949. DOI: 10.1016/j.aninu.2022.09.003. View

13.

Roager H, Licht T . Microbial tryptophan catabolites in health and disease. Nat Commun. 2018; 9(1):3294. PMC: 6098093. DOI: 10.1038/s41467-018-05470-4. View

14.

Figueroa J, Lewis A, Miller P, Fischer R, Diedrichsen R . Growth, carcass traits, and plasma amino acid concentrations of gilts fed low-protein diets supplemented with amino acids including histidine, isoleucine, and valine. J Anim Sci. 2003; 81(6):1529-37. DOI: 10.2527/2003.8161529x. View

15.

Wu G . Functional amino acids in nutrition and health. Amino Acids. 2013; 45(3):407-11. DOI: 10.1007/s00726-013-1500-6. View

16.

Davila A, Blachier F, Gotteland M, Andriamihaja M, Benetti P, Sanz Y . Intestinal luminal nitrogen metabolism: role of the gut microbiota and consequences for the host. Pharmacol Res. 2012; 68(1):95-107. DOI: 10.1016/j.phrs.2012.11.005. View

17.

Zheng L, Wei H, Cheng C, Xiang Q, Pang J, Peng J . Supplementation of branched-chain amino acids to a reduced-protein diet improves growth performance in piglets: involvement of increased feed intake and direct muscle growth-promoting effect. Br J Nutr. 2016; 115(12):2236-45. DOI: 10.1017/S0007114516000842. View

18.

Russell L, Kerr B, Easter R . Limiting amino acids in an 11% crude protein corn-soybean meal diet for growing pigs. J Anim Sci. 1987; 65(5):1266-72. DOI: 10.2527/jas1987.6551266x. View

19.

Kubota K, Hayashi M, Matsunaga K, Iguchi A, Ohashi A, Li Y . Microbial community composition of a down-flow hanging sponge (DHS) reactor combined with an up-flow anaerobic sludge blanket (UASB) reactor for the treatment of municipal sewage. Bioresour Technol. 2013; 151:144-50. DOI: 10.1016/j.biortech.2013.10.058. View

20.

Jiao X, Ma W, Chen Y, Li Z . Effects of amino acids supplementation in low crude protein diets on growth performance, carcass traits and serum parameters in finishing gilts. Anim Sci J. 2016; 87(10):1252-1257. DOI: 10.1111/asj.12542. View