MiR-21 Regulates Growth and Migration of Cervical Cancer Cells by RECK Signaling Pathway

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Apr 13

PMID 38612895

Authors

Seidy Y Aguilar-Martinez

Gabriela E Campos-Viguri

Selma E Medina-Garcia

Ricardo J Garcia-Flores

Jessica Deas

Claudia Gomez-Ceron

Abraham Pedroza-Torres

Elizabeth Bautista-Rodriguez

Gloria Fernandez-Tilapa

Mauricio Rodriguez-Dorantes

Carlos Perez-Plasencia

Oscar Peralta-Zaragoza

Affiliations

Soon will be listed here.

Abstract

Expression of miR-21 has been found to be altered in almost all types of cancers, and it has been classified as an oncogenic microRNA. In addition, the expression of tumor suppressor gene RECK is associated with miR-21 overexpression in high-grade cervical lesions. In the present study, we analyze the role of miR-21 in RECK gene regulation in cervical cancer cells. To identify the downstream cellular target genes of upstream miR-21, we silenced endogenous miR-21 expression using siRNAs. We analyzed the expression of miR-21 and RECK, as well as functional effects on cell proliferation and migration. We found that in cervical cancer cells, there was an inverse correlation between miR-21 expression and RECK mRNA and protein expression. SiRNAs to miR-21 increased luciferase reporter activity in construct plasmids containing the RECK-3'-UTR microRNA response elements MRE21-1, MRE21-2, and MRE21-3. The role of miR-21 in cell proliferation was also analyzed, and cancer cells transfected with siRNAs exhibited a markedly reduced cell proliferation and migration. Our findings indicate that miR-21 post-transcriptionally down-regulates the expression of RECK to promote cell proliferation and cell migration inhibition in cervical cancer cell survival. Therefore, miR-21 and RECK may be potential therapeutic targets in gene therapy for cervical cancer.

Citing Articles

miR-21 and miR-145 as Prognostic Biomarkers for Radiotherapy Responses in Cervical Cancer Patients: A Preliminary Study.

Putra A, Andrijono , Winarto H, Prijanti A, Rachmadi L, Pakasi T Int J Mol Sci. 2024; 25(19).

PMID: 39408872 PMC: 11477450. DOI: 10.3390/ijms251910545.

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