Oxidative Stress Markers and Histopathological Changes in Selected Organs of Mice Infected with Murine Norovirus 1 (MNV-1)

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Apr 13

PMID 38612426

Authors

Paulina Janicka

Dominika Stygar

Elzbieta Chelmecka

Piotr Kuropka

Arkadiusz Miazek

Aleksandra Studzinska

Aleksandra Pogorzelska

Katarzyna Pala

Barbara Bazanow

Affiliations

Soon will be listed here.

Abstract

This paper describes the effects of murine norovirus (MNV) infection on oxidative stress and histopathological changes in mice. This study uses histopathological assays, enzymatic and non-enzymatic antioxidant markers, and total oxidative status and capacity (TOS, TAC). The results suggest that MNV infection can lead to significant changes with respect to the above-mentioned parameters in various organs. Specifically, reduced superoxide dismutase (SOD), Mn superoxide dismutase (MnSOD), catalase (CAT), and glutathione reductase (GR) activities were observed in liver tissues, while higher MnSOD activity was observed in kidney tissues of MNV-infected mice when compared to the control. GR activity was lower in all tissues of MNV-infected mice tested, with the exception of lung tissue. This study also showed that norovirus infection led to increased TOS levels in the brain and liver and TAC levels in the brain, while TOS levels were significantly reduced in the kidneys. These changes may be due to the production of reactive oxygen species (ROS) caused by the viral infection. ROS can damage cells and contribute to oxidative stress. These studies help us to understand the pathogenesis of MNV infection and its potential effects on oxidative stress and histopathological changes in mice, and pave the way for further studies of the long-term effects of MNV infection.

References

Basic M, Keubler L, Buettner M, Achard M, Breves G, Schroder B . Norovirus triggered microbiota-driven mucosal inflammation in interleukin 10-deficient mice. Inflamm Bowel Dis. 2014; 20(3):431-43. DOI: 10.1097/01.MIB.0000441346.86827.ed. View

Oyanagui Y . Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem. 1984; 142(2):290-6. DOI: 10.1016/0003-2697(84)90467-6. View

Kahan S, Liu G, Reinhard M, Hsu C, Livingston R, Karst S . Comparative murine norovirus studies reveal a lack of correlation between intestinal virus titers and enteric pathology. Virology. 2011; 421(2):202-10. PMC: 3210872. DOI: 10.1016/j.virol.2011.09.030. View

Mumphrey S, Changotra H, Moore T, Heimann-Nichols E, Wobus C, Reilly M . Murine norovirus 1 infection is associated with histopathological changes in immunocompetent hosts, but clinical disease is prevented by STAT1-dependent interferon responses. J Virol. 2007; 81(7):3251-63. PMC: 1866040. DOI: 10.1128/JVI.02096-06. View

Van Dycke J, Neyts J, Rocha-Pereira J . Assessing the Efficacy of Small Molecule Inhibitors in aMouse Model of Persistent Norovirus Infection. Bio Protoc. 2021; 8(9):e2831. PMC: 8275229. DOI: 10.21769/BioProtoc.2831. View

Fernandes I, de Brito C, Dos Reis V, Sato M, Pereira N . SARS-CoV-2 and Other Respiratory Viruses: What Does Oxidative Stress Have to Do with It?. Oxid Med Cell Longev. 2020; 2020:8844280. PMC: 7757116. DOI: 10.1155/2020/8844280. View

Erel O . A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004; 37(4):277-85. DOI: 10.1016/j.clinbiochem.2003.11.015. View

Abedi A, Ghobadi H, Sharghi A, Iranpour S, Fazlzadeh M, Aslani M . Effect of saffron supplementation on oxidative stress markers (MDA, TAC, TOS, GPx, SOD, and pro-oxidant/antioxidant balance): An updated systematic review and meta-analysis of randomized placebo-controlled trials. Front Med (Lausanne). 2023; 10:1071514. PMC: 9928952. DOI: 10.3389/fmed.2023.1071514. View

Cecchini R, Cecchini A . SARS-CoV-2 infection pathogenesis is related to oxidative stress as a response to aggression. Med Hypotheses. 2020; 143:110102. PMC: 7357498. DOI: 10.1016/j.mehy.2020.110102. View

10.

Hsu C, Piotrowski S, Meeker S, Smith K, Maggio-Price L, Treuting P . Histologic Lesions Induced by Murine Norovirus Infection in Laboratory Mice. Vet Pathol. 2016; 53(4):754-63. PMC: 5586550. DOI: 10.1177/0300985815618439. View

11.

Halliwell B . Free radicals and antioxidants: updating a personal view. Nutr Rev. 2012; 70(5):257-65. DOI: 10.1111/j.1753-4887.2012.00476.x. View

12.

Garofalo R, Kolli D, Casola A . Respiratory syncytial virus infection: mechanisms of redox control and novel therapeutic opportunities. Antioxid Redox Signal. 2012; 18(2):186-217. PMC: 3513983. DOI: 10.1089/ars.2011.4307. View

13.

Hsu C, Wobus C, Steffen E, Riley L, Livingston R . Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice. Clin Diagn Lab Immunol. 2005; 12(10):1145-51. PMC: 1247840. DOI: 10.1128/CDLI.12.10.1145-1151.2005. View

14.

AEBI H . Catalase in vitro. Methods Enzymol. 1984; 105:121-6. DOI: 10.1016/s0076-6879(84)05016-3. View

15.

Wobus C, Thackray L, Virgin 4th H . Murine norovirus: a model system to study norovirus biology and pathogenesis. J Virol. 2006; 80(11):5104-12. PMC: 1472167. DOI: 10.1128/JVI.02346-05. View

16.

Kim J, Seok S, Kim D, Baek M, Na Y, Han J . Prevalence of murine norovirus infection in Korean laboratory animal facilities. J Vet Med Sci. 2010; 73(5):687-91. DOI: 10.1292/jvms.10-0226. View

17.

Mannervik B . Glutathione peroxidase. Methods Enzymol. 1985; 113:490-5. DOI: 10.1016/s0076-6879(85)13063-6. View

18.

Kelmenson J, Pomerleau D, Griffey S, Zhang W, Karolak M, Fahey J . Kinetics of transmission, infectivity, and genome stability of two novel mouse norovirus isolates in breeding mice. Comp Med. 2009; 59(1):27-36. PMC: 2703142. View

19.

Tsikas D . Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Anal Biochem. 2016; 524:13-30. DOI: 10.1016/j.ab.2016.10.021. View

20.

Ntyonga-Pono M . COVID-19 infection and oxidative stress: an under-explored approach for prevention and treatment?. Pan Afr Med J. 2020; 35(Suppl 2):12. PMC: 7266475. DOI: 10.11604/pamj.2020.35.2.22877. View