Comparative Transcriptome Analysis of the Hepatopancreas from Exposed to the Heavy Metal Copper

Overview

Journal Animals (Basel)

Date 2024 Apr 13

PMID 38612356

Authors

Jiayuan Zhang

Zhiming Bao

Jieyu Guo

Xianbin Su

Yongfeng Zou

Hui Guo

Affiliations

Soon will be listed here.

Abstract

The contamination of aquatic ecosystems by the heavy metal copper (Cu) is an important environmental issue and poses significant risks to the physiological functions of aquatic organisms. is one of the most important freshwater-cultured prawns in the world. The hepatopancreas of crustaceans is a key organ for immune defense, heavy metal accumulation, and detoxification, playing a pivotal role in toxicological research. However, research on the molecular response of the hepatopancreas in to Cu exposure is still lacking. In this study, the transcriptomic response in the hepatopancreas of was studied after Cu exposure for 3 and 48 h. Compared with the control group, 11,164 (7288 up-regulated and 3876 down-regulated genes) and 10,937 (6630 up-regulated and 4307 down-regulated genes) differentially expressed genes (DEGs) were identified after 3 and 48 h exposure, respectively. Most of these DEGs were up-regulated, implying that gene expressions were largely induced by Cu. Functional enrichment analysis of these DEGs revealed that immunity, copper homeostasis, detoxification, DNA damage repair, and apoptosis were differentially regulated by Cu. Seven genes involved in immunity, detoxification, and metabolism were selected for validation by qRT-PCR, and the results confirmed the reliability of RNA-Seq. All these findings suggest that attempts to resist the toxicity of Cu by up-regulating the expression of genes related to immunity, metabolism, and detoxification. However, with the excessive accumulation of reactive oxygen species (ROS), the antioxidant enzyme system was destroyed. As a result, DNA damage repair and the cellular stress response were inhibited, thereby exacerbating cell damage. In order to maintain the normal function of the hepatopancreas, removes damaged cells by activating the apoptosis mechanism. Our study not only facilitates an understanding of the molecular response mechanisms of underlying Cu toxicity effects but also helps us to identify potential biomarkers associated with the stress response in other crustaceans.

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References

Zhou T, Liu J, Chan S, Wang W . Molecular characterization and expression dynamics of three key genes in the PI3K-AKT pathway reveal its involvement in the immunotoxicological responses of the giant river prawn Macrobrachium rosenbergii to acute ammonia and nitrite stress. Ecotoxicol Environ Saf. 2021; 208:111767. DOI: 10.1016/j.ecoenv.2020.111767. View

Zhao D, Zhang X, Liu D, Ru S . Cu accumulation, detoxification and tolerance in the red swamp crayfish Procambarus clarkii. Ecotoxicol Environ Saf. 2019; 175:201-207. DOI: 10.1016/j.ecoenv.2019.03.031. View

Guo H, Li K, Wang W, Wang C, Shen Y . Effects of Copper on Hemocyte Apoptosis, ROS Production, and Gene Expression in White Shrimp Litopenaeus vannamei. Biol Trace Elem Res. 2017; 179(2):318-326. DOI: 10.1007/s12011-017-0974-6. View

Bian C, Zheng Z, Su J, Chang S, Yu H, Bao J . Copper homeostasis and cuproptosis in tumor pathogenesis and therapeutic strategies. Front Pharmacol. 2023; 14:1271613. PMC: 10520736. DOI: 10.3389/fphar.2023.1271613. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Thiel T, Michalek W, Varshney R, Graner A . Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.). Theor Appl Genet. 2003; 106(3):411-22. DOI: 10.1007/s00122-002-1031-0. View

Ruan Z, Wan Z, Yang L, Li W, Wang Q . JAK/STAT signalling regulates antimicrobial activities in Eriocheir sinensis. Fish Shellfish Immunol. 2018; 84:491-501. DOI: 10.1016/j.fsi.2018.09.059. View

Wei H, Wang S, Xu E, Liu J, Li X, Wang Z . Synergistic toxicity of microcystin-LR and Cu to zebrafish (Danio rerio). Sci Total Environ. 2020; 713:136393. DOI: 10.1016/j.scitotenv.2019.136393. View

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

10.

Dawood M . Dietary Copper Requirements for Aquatic Animals: A Review. Biol Trace Elem Res. 2022; 200(12):5273-5282. DOI: 10.1007/s12011-021-03079-1. View

11.

Li Y, Yuan W, Xu Q, Liu H, Dai X . The regulation of immune responses against white spot syndrome virus or Vibrio alginolyticus in toll-like receptors silenced giant freshwater prawn (Macrobrachium rosenbergii). Fish Shellfish Immunol. 2020; 107(Pt A):84-94. DOI: 10.1016/j.fsi.2020.10.003. View

12.

Luo J, Chen Y, Huang Y, Feng J, Yuan Y, Jian J . A novel C-type lectin for Litopenaeus vannamei involved in the innate immune response against Vibrio infection. Fish Shellfish Immunol. 2023; 135:108621. DOI: 10.1016/j.fsi.2023.108621. View

13.

Tang D, Liu R, Shi X, Shen C, Bai Y, Tang B . Toxic effects of metal copper stress on immunity, metabolism and pathologic changes in Chinese mitten crab (Eriocheir japonica sinensis). Ecotoxicology. 2021; 30(4):632-642. DOI: 10.1007/s10646-021-02367-9. View

14.

Li N, Ma Q, Yang J, Zhao Y, Brochon J . Effects of water-borne copper on the gills and hepatopancreas of Macrobrachium rosenbergii. Integr Zool. 2011; 3(4):299-310. DOI: 10.1111/j.1749-4877.2008.00094.x. View

15.

Zhang X, Tang N, Hadden T, Rishi A . Akt, FoxO and regulation of apoptosis. Biochim Biophys Acta. 2011; 1813(11):1978-86. DOI: 10.1016/j.bbamcr.2011.03.010. View

16.

Liu K, Yeh M, Kou G, Cheng W, Lo C . Identification and cloning of a selenium-dependent glutathione peroxidase from tiger shrimp, Penaeus monodon, and its transcription following pathogen infection and related to the molt stages. Dev Comp Immunol. 2010; 34(9):935-44. DOI: 10.1016/j.dci.2010.04.001. View

17.

Zhang Z, Zhang C, Dai X, Zhang R, Cao X, Wang K . Two relish isoforms produced by alternative splicing participate in the regulation of antimicrobial peptides expression in Procambarus clarkii intestine. Fish Shellfish Immunol. 2020; 99:107-118. DOI: 10.1016/j.fsi.2020.02.003. View

18.

Marifah F, Saputri M, Soegianto A, Irawan B, Putranto T . The Change of Metallothionein and Oxidative Response in Gills of the after Exposure to Copper. Animals (Basel). 2019; 9(6). PMC: 6616881. DOI: 10.3390/ani9060353. View

19.

Coates C, Decker H . Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin. Cell Mol Life Sci. 2016; 74(2):293-317. PMC: 5219038. DOI: 10.1007/s00018-016-2326-7. View

20.

Xing K, Liu Y, Yan C, Zhou Y, Sun Y, Su N . Transcriptome analysis of Neocaridina denticulate sinensis under copper exposure. Gene. 2020; 764:145098. DOI: 10.1016/j.gene.2020.145098. View