DNA Damage Response and Cell Cycle Regulation in Bacteria: a Twist Around the Paradigm

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Apr 12

PMID 38605710

Authors

Hari Sharan Misra

Yogendra Singh Rajpurohit

Affiliations

Soon will be listed here.

Abstract

The co-protease activity in the RecA-ssDNA complex cleaves the autorepressor LexA, resulting in the derepression of a large number of genes under LexA control. This process is called the SOS response, and genes that are expressed in response to DNA damage are called SOS genes. The proteins encoded by the SOS genes are involved in both DNA repair and maintaining the functions of crucial cell division proteins (e.g., FtsZ) under check until the damaged DNA is presumably repaired. This mechanism of SOS response is the only known mechanism of DNA damage response and cell cycle regulation in bacteria. However, there are bacteria that do not obey this rule of DNA damage response and cell cycle regulation, yet they respond to DNA damage, repair it, and survive. That means such bacteria would have some alternate mechanism(s) of DNA damage response and cell cycle regulation beyond the canonical pathway of the SOS response. In this study, we present the perspectives that bacteria may have other mechanisms of DNA damage response and cell cycle regulation mediated by bacterial eukaryotic type Ser/Thr protein kinases as an alternate to the canonical SOS response and herewith elaborate on them with a well-studied example in the radioresistant bacterium .

Citing Articles

Surviving the storm: exploring the role of natural transformation in nutrition and DNA repair of stressed .

Sharma D, Soni I, Rajpurohit Y Appl Environ Microbiol. 2024; 91(1):e0137124.

PMID: 39651863 PMC: 11784314. DOI: 10.1128/aem.01371-24.

References

Wang Y, Xu Q, Lu H, Lin L, Wang L, Xu H . Protease activity of PprI facilitates DNA damage response: Mn2+-dependence and substrate sequence-specificity of the proteolytic reaction. PLoS One. 2015; 10(3):e0122071. PMC: 4374696. DOI: 10.1371/journal.pone.0122071. View

Blanchard L, Guerin P, Roche D, Cruveiller S, Pignol D, Vallenet D . Conservation and diversity of the IrrE/DdrO-controlled radiation response in radiation-resistant Deinococcus bacteria. Microbiologyopen. 2017; 6(4). PMC: 5552922. DOI: 10.1002/mbo3.477. View

Mukherjee A, Cao C, Lutkenhaus J . Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli. Proc Natl Acad Sci U S A. 1998; 95(6):2885-90. PMC: 19664. DOI: 10.1073/pnas.95.6.2885. View

Kota S, Dhamodharan V, Pradeepkumar P, Misra H . G-quadruplex forming structural motifs in the genome of Deinococcus radiodurans and their regulatory roles in promoter functions. Appl Microbiol Biotechnol. 2015; 99(22):9761-9. DOI: 10.1007/s00253-015-6808-6. View

Rajpurohit Y, Misra H . Structure-function study of deinococcal serine/threonine protein kinase implicates its kinase activity and DNA repair protein phosphorylation roles in radioresistance of Deinococcus radiodurans. Int J Biochem Cell Biol. 2013; 45(11):2541-52. DOI: 10.1016/j.biocel.2013.08.011. View

Courcelle J, Khodursky A, Peter B, Brown P, HANAWALT P . Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli. Genetics. 2001; 158(1):41-64. PMC: 1461638. DOI: 10.1093/genetics/158.1.41. View

Maurya G, Modi K, Banerjee M, Chaudhary R, Rajpurohit Y, Misra H . Phosphorylation of FtsZ and FtsA by a DNA Damage-Responsive Ser/Thr Protein Kinase Affects Their Functional Interactions in . mSphere. 2018; 3(4). PMC: 6052341. DOI: 10.1128/mSphere.00325-18. View

Khan F, Singh S, Mishra B . Conservation of the LexA repressor binding site in Deinococcus radiodurans. J Integr Bioinform. 2010; 5(1). DOI: 10.2390/biecoll-jib-2008-86. View

Devigne A, Ithurbide S, Bouthier de la Tour C, Passot F, Mathieu M, Sommer S . DdrO is an essential protein that regulates the radiation desiccation response and the apoptotic-like cell death in the radioresistant Deinococcus radiodurans bacterium. Mol Microbiol. 2015; 96(5):1069-84. DOI: 10.1111/mmi.12991. View

10.

Narumi I, Satoh K, Kikuchi M, Funayama T, Yanagisawa T, Kobayashi Y . The LexA protein from Deinococcus radiodurans is not involved in RecA induction following gamma irradiation. J Bacteriol. 2001; 183(23):6951-6. PMC: 95538. DOI: 10.1128/JB.183.23.6951-6956.2001. View

11.

Narasimha A, Basu B . New insights into the activation of Radiation Desiccation Response regulon in . J Biosci. 2021; 46. View

12.

Kota S, Charaka V, Ringgaard S, Waldor M, Misra H . PprA contributes to Deinococcus radiodurans resistance to nalidixic acid, genome maintenance after DNA damage and interacts with deinococcal topoisomerases. PLoS One. 2014; 9(1):e85288. PMC: 3893189. DOI: 10.1371/journal.pone.0085288. View

13.

Chaudhary R, Mishra S, Maurya G, Rajpurohit Y, Misra H . FtsZ phosphorylation brings about growth arrest upon DNA damage in . FASEB Bioadv. 2023; 5(1):27-42. PMC: 9832530. DOI: 10.1096/fba.2022-00082. View

14.

Narumi I, Satoh K, Cui S, Funayama T, Kitayama S, Watanabe H . PprA: a novel protein from Deinococcus radiodurans that stimulates DNA ligation. Mol Microbiol. 2004; 54(1):278-85. DOI: 10.1111/j.1365-2958.2004.04272.x. View

15.

Murakami M, Narumi I, Satoh K, Furukawa A, Hayata I . Analysis of interaction between DNA and Deinococcus radiodurans PprA protein by atomic force microscopy. Biochim Biophys Acta. 2005; 1764(1):20-3. DOI: 10.1016/j.bbapap.2005.10.017. View

16.

Chen Y, Milam S, Erickson H . SulA inhibits assembly of FtsZ by a simple sequestration mechanism. Biochemistry. 2012; 51(14):3100-9. PMC: 3518438. DOI: 10.1021/bi201669d. View

17.

Rajpurohit Y, Sharma D, Misra H . Involvement of serine / threonine protein kinases in DNA damage response and cell division in bacteria. Res Microbiol. 2021; 173(1-2):103883. DOI: 10.1016/j.resmic.2021.103883. View

18.

Joshi B, Schmid R, Altendorf K, Apte S . Protein recycling is a major component of post-irradiation recovery in Deinococcus radiodurans strain R1. Biochem Biophys Res Commun. 2004; 320(4):1112-7. DOI: 10.1016/j.bbrc.2004.06.062. View

19.

Jolivet E, Lecointe F, Coste G, Satoh K, Narumi I, Bailone A . Limited concentration of RecA delays DNA double-strand break repair in Deinococcus radiodurans R1. Mol Microbiol. 2005; 59(1):338-49. DOI: 10.1111/j.1365-2958.2005.04946.x. View

20.

Sharma D, Bihani S, Siddiqui M, Misra H, Rajpurohit Y . WD40 domain of RqkA regulates its kinase activity and role in extraordinary radioresistance of . J Biomol Struct Dyn. 2020; 40(3):1246-1259. DOI: 10.1080/07391102.2020.1824810. View