Self-assembly of a AuNPs/TiC MXene Hydrogel for Cascade Amplification of MicroRNA-122 Biosensing

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2024 Apr 11

PMID 38605266

Authors

Xiao Yang

Chunguang Li

Jianfei Xia

Feifei Zhang

Zonghua Wang

Affiliations

Soon will be listed here.

Abstract

A three-dimensional (3D) self-assembled AuNPs/TiC MXene hydrogel (AuNPs/TiC MXH) nanocomposite was prepared for the fabrication of a novel microRNA-122 electrochemical biosensor. The 3D hydrogel structure was gelated from two-dimensional MXene nanosheets with the assistance of graphite oxide and ethylenediamine. MXene hydrogels supported the in situ formation of Au nanoparticles (AuNPs) that predominantly exploring the (111) facet, and these AuNPs are utilized as carriers for hairpin DNA (hpDNA) probes, facilitating DNA hybridization. MXene acted as both a reductant and stabilizer, significantly improving the electrochemical signal. In addition, the conjugation of PAMAM dendrimer-encapsulated AuNPs and H-DNA worked as an ideal bridge to connect targets and efficient electrochemical tags, providing a high amplification efficiency for the sensing of microRNA-122. A linear relationship between the peak currents and the logarithm of the concentrations of microRNA-122 from 1.0 × 10 to 1.0 × 10 fM (I = 1.642 + 0.312 lgc, R = 0.9891), is obtained. The detection limit is 0.8 × 10 fM (S/N = 3). The average recovery for human serum detection ranged from 97.32 to 101.4% (RSD < 5%).

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