Preparation of Functional Poly-(lactic Acid-co-glycolic Acid)-based Guided Bone-regeneration Membrane and Its Application in the Reconstruction of Mandibular Defects in Rats

Overview

Journal Hua Xi Kou Qiang Yi Xue Za Zhi

Specialty Dentistry

Date 2024 Apr 10

PMID 38596972

Authors

Yiming Liu

Yun Zhao

Mei Han

Yuqiu Zhang

Fanglin Mi

Bing Wang

Affiliations

Soon will be listed here.

Abstract

Objectives: This study aimed to prepare functional composite electrospinning fibrous membranes with the functions of antifibrosis and bacteriostasis, as well as to explore its repair effect on rat jaw defect.

Methods: Poly-(lactic acid-co-glycolic acid) fibrous membrane loaded with cyclic-arginine-glycine-aspartic acid sequence, ornidazole, and nanohydroxyapatite (n-HA) was prepared by electrospinning as the functional layer (GBRL) for adhering onto defective bone. A barrier layer with the function of supporting and isolating different functional layers was prepared by tape-casting method. Poly(p-dioxanone-co-l-phenylalanine) fiber membrane with the function of inhibiting fibrosis was prepared by electrospinning technology as the antifibrosis layer (AFL). The morphology of the composite membrane was characterized by scanning electron microscopy. The effects of different functional layers on the proliferation of mouse osteoblast precursor cells (MC3T3-E1) and mouse fibroblasts (L929) were studied by cell-proliferation test (CCK-8 method). The inhibitory effect of composite membrane on the proliferation of was studied by bacteriostatic circle test. A rat mandibular-bone defect model was established to study the repair effect of composite fiber membrane on bone-defect tissue. This repair effect was compared with that of collagen oral-repair membrane commonly used in clinics.

Results: The GBRL layer could support the proliferation of MC3T3-E1 cells, and the AFL layer could inhibit the proliferation of L929 cells. Composite membranes loaded with ornidazole could inhibit the proliferation of . Various composite membranes can induce the reconstruction of rat jaw defects, among which the composite membranes loaded with ornidazole and n-HA had the best repair effect, which was better than that of collagen oral-repair membrane.

Conclusions: The electrospun membrane loaded with ornidazole and n-HA as the composite fiber membrane of GBRL layer had excellent antibacterial and bone-tissue-regeneration activity. The effect was better than that of the commonly used collagen oral-repair membrane. Therefore, this material has great potential to induce bone regeneration for defects caused by periodontal diseases.

References

Choi C, Xu Y, Wang B, Zhu M, Zhang L, Bian L . Substrate Coupling Strength of Integrin-Binding Ligands Modulates Adhesion, Spreading, and Differentiation of Human Mesenchymal Stem Cells. Nano Lett. 2015; 15(10):6592-600. DOI: 10.1021/acs.nanolett.5b02323. View

Qasim S, Najeeb S, Delaine-Smith R, Rawlinson A, Rehman I . Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration. Dent Mater. 2016; 33(1):71-83. DOI: 10.1016/j.dental.2016.10.003. View

Luo K, Gao X, Gao Y, Li Y, Deng M, Tan J . Multiple integrin ligands provide a highly adhesive and osteoinductive surface that improves selective cell retention technology. Acta Biomater. 2018; 85:106-116. DOI: 10.1016/j.actbio.2018.12.018. View

Abe G, Sasaki J, Katata C, Kohno T, Tsuboi R, Kitagawa H . Fabrication of novel poly(lactic acid/caprolactone) bilayer membrane for GBR application. Dent Mater. 2020; 36(5):626-634. DOI: 10.1016/j.dental.2020.03.013. View

Cannella V, Altomare R, Chiaramonte G, Di Bella S, Mira F, Russotto L . Cytotoxicity Evaluation of Endodontic Pins on L929 Cell Line. Biomed Res Int. 2019; 2019:3469525. PMC: 6877943. DOI: 10.1155/2019/3469525. View

Wang B, Wen A, Feng C, Niu L, Xiao X, Luo L . The in vivo anti-fibrotic function of calcium sensitive receptor (CaSR) modulating poly(p-dioxanone-co-l-phenylalanine) prodrug. Acta Biomater. 2018; 73:180-189. DOI: 10.1016/j.actbio.2018.04.018. View

Watcharajittanont N, Tabrizian M, Putson C, Pripatnanont P, Meesane J . Osseointegrated membranes based on electro-spun TiO/hydroxyapatite/polyurethane for oral maxillofacial surgery. Mater Sci Eng C Mater Biol Appl. 2020; 108:110479. DOI: 10.1016/j.msec.2019.110479. View

Sudo H, Kodama H, Amagai Y, Yamamoto S, Kasai S . In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 1983; 96(1):191-8. PMC: 2112252. DOI: 10.1083/jcb.96.1.191. View

Steigmann L, Jung O, Kieferle W, Stojanovic S, Proehl A, Gorke O . Biocompatibility and Immune Response of a Newly Developed Volume-Stable Magnesium-Based Barrier Membrane in Combination with a PVD Coating for Guided Bone Regeneration (GBR). Biomedicines. 2021; 8(12). PMC: 7767206. DOI: 10.3390/biomedicines8120636. View

10.

Krishnan V, Dhurjati R, Vogler E, Mastro A . Osteogenesis in vitro: from pre-osteoblasts to osteocytes: a contribution from the Osteobiology Research Group, The Pennsylvania State University. In Vitro Cell Dev Biol Anim. 2009; 46(1):28-35. DOI: 10.1007/s11626-009-9238-x. View

11.

Nemati S, Kim S, Shin Y, Shin H . Current progress in application of polymeric nanofibers to tissue engineering. Nano Converg. 2019; 6(1):36. PMC: 6838281. DOI: 10.1186/s40580-019-0209-y. View

12.

Wang Z, Ma K, Jiang X, Xie J, Cai P, Li F . Electrospun poly(3-hydroxybutyrate-co-4-hydroxybutyrate) /Octacalcium phosphate Nanofibrous membranes for effective guided bone regeneration. Mater Sci Eng C Mater Biol Appl. 2020; 112:110763. DOI: 10.1016/j.msec.2020.110763. View

13.

Sartika D, Wang C, Wang D, Cherng J, Chang S, Fan G . Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration. Polymers (Basel). 2020; 12(4). PMC: 7240549. DOI: 10.3390/polym12040853. View

14.

Barbeck M, Kuhnel L, Witte F, Pissarek J, Precht C, Xiong X . Degradation, Bone Regeneration and Tissue Response of an Innovative Volume Stable Magnesium-Supported GBR/GTR Barrier Membrane. Int J Mol Sci. 2020; 21(9). PMC: 7247710. DOI: 10.3390/ijms21093098. View

15.

Liu X, He X, Jin D, Wu S, Wang H, Yin M . A biodegradable multifunctional nanofibrous membrane for periodontal tissue regeneration. Acta Biomater. 2020; 108:207-222. DOI: 10.1016/j.actbio.2020.03.044. View

16.

George A, Sanjay M, Srisuk R, Parameswaranpillai J, Siengchin S . A comprehensive review on chemical properties and applications of biopolymers and their composites. Int J Biol Macromol. 2020; 154:329-338. DOI: 10.1016/j.ijbiomac.2020.03.120. View

17.

Luo J, Zhu J, Wang L, Kang J, Wang X, Xiong J . Co-electrospun nano-/microfibrous composite scaffolds with structural and chemical gradients for bone tissue engineering. Mater Sci Eng C Mater Biol Appl. 2020; 119:111622. DOI: 10.1016/j.msec.2020.111622. View

18.

Xu Y, Zhao S, Weng Z, Zhang W, Wan X, Cui T . Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties. ACS Appl Mater Interfaces. 2020; 12(49):54497-54506. DOI: 10.1021/acsami.0c18070. View

19.

Zhang L, Dong Y, Zhang N, Shi J, Zhang X, Qi C . Potentials of sandwich-like chitosan/polycaprolactone/gelatin scaffolds for guided tissue regeneration membrane. Mater Sci Eng C Mater Biol Appl. 2020; 109:110618. DOI: 10.1016/j.msec.2019.110618. View

20.

Peres M, Macpherson L, Weyant R, Daly B, Venturelli R, Mathur M . Oral diseases: a global public health challenge. Lancet. 2019; 394(10194):249-260. DOI: 10.1016/S0140-6736(19)31146-8. View