Engineered Exosomes in Emerging Cell-free Therapy

Overview

Journal Front Oncol

Specialty Oncology

Date 2024 Apr 10

PMID 38595822

Authors

Chaohua Si

Jianen Gao

Xu Ma

Affiliations

Soon will be listed here.

Abstract

The discovery and use of exosomes ushered in a new era of cell-free therapy. Exosomes are a subgroup of extracellular vesicles that show great potential in disease treatment. Engineered exosomes. with their improved functions have attracted intense interests of their application in translational medicine research. However, the technology of engineering exosomes still faces many challenges which have been the great limitation for their clinical application. This review summarizes the current status of research on engineered exosomes and the difficulties encountered in recent years, with a view to providing new approaches and ideas for future exosome modification and new drug development.

Citing Articles

Advances and challenges in the use of liquid biopsy in gynaecological oncology.

Zhang Y, Tian L Heliyon. 2024; 10(20):e39148.

PMID: 39492906 PMC: 11530831. DOI: 10.1016/j.heliyon.2024.e39148.

Roles of M1 Macrophages and Their Extracellular Vesicles in Cancer Therapy.

Zhou W, Yang F, Zhang X Cells. 2024; 13(17.

PMID: 39273000 PMC: 11394047. DOI: 10.3390/cells13171428.

Therapeutic potential of extracellular vesicles from diverse sources in cancer treatment.

Lin H, Zhou J, Ding T, Zhu Y, Wang L, Zhong T Eur J Med Res. 2024; 29(1):350.

PMID: 38943222 PMC: 11212438. DOI: 10.1186/s40001-024-01937-x.

References

Zhang L, Yu D . Exosomes in cancer development, metastasis, and immunity. Biochim Biophys Acta Rev Cancer. 2019; 1871(2):455-468. PMC: 6542596. DOI: 10.1016/j.bbcan.2019.04.004. View

Dougherty J, Kumar N, Noor M, Angelos M, Khan M, Chen C . Extracellular Vesicles Released by Human Induced-Pluripotent Stem Cell-Derived Cardiomyocytes Promote Angiogenesis. Front Physiol. 2019; 9:1794. PMC: 6302004. DOI: 10.3389/fphys.2018.01794. View

Hu S, Ma J, Su C, Chen Y, Shu Y, Qi Z . Engineered exosome-like nanovesicles suppress tumor growth by reprogramming tumor microenvironment and promoting tumor ferroptosis. Acta Biomater. 2021; 135:567-581. DOI: 10.1016/j.actbio.2021.09.003. View

Hu Z, Chen G, Zhao Y, Gao H, Li L, Yin Y . Exosome-derived circCCAR1 promotes CD8 + T-cell dysfunction and anti-PD1 resistance in hepatocellular carcinoma. Mol Cancer. 2023; 22(1):55. PMC: 10024440. DOI: 10.1186/s12943-023-01759-1. View

Skog J, Wurdinger T, van Rijn S, Meijer D, Gainche L, Sena-Esteves M . Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol. 2008; 10(12):1470-6. PMC: 3423894. DOI: 10.1038/ncb1800. View

Shi X, Cheng Q, Hou T, Han M, Smbatyan G, Lang J . Genetically Engineered Cell-Derived Nanoparticles for Targeted Breast Cancer Immunotherapy. Mol Ther. 2019; 28(2):536-547. PMC: 7001084. DOI: 10.1016/j.ymthe.2019.11.020. View

Luga V, Zhang L, Viloria-Petit A, Ogunjimi A, Inanlou M, Chiu E . Exosomes mediate stromal mobilization of autocrine Wnt-PCP signaling in breast cancer cell migration. Cell. 2012; 151(7):1542-56. DOI: 10.1016/j.cell.2012.11.024. View

Zhu L, Kalimuthu S, Gangadaran P, Oh J, Lee H, Baek S . Exosomes Derived From Natural Killer Cells Exert Therapeutic Effect in Melanoma. Theranostics. 2017; 7(10):2732-2745. PMC: 5558565. DOI: 10.7150/thno.18752. View

Pathan M, Fonseka P, Chitti S, Kang T, Sanwlani R, Van Deun J . Vesiclepedia 2019: a compendium of RNA, proteins, lipids and metabolites in extracellular vesicles. Nucleic Acids Res. 2018; 47(D1):D516-D519. PMC: 6323905. DOI: 10.1093/nar/gky1029. View

10.

Ridder K, Keller S, Dams M, Rupp A, Schlaudraff J, Del Turco D . Extracellular vesicle-mediated transfer of genetic information between the hematopoietic system and the brain in response to inflammation. PLoS Biol. 2014; 12(6):e1001874. PMC: 4043485. DOI: 10.1371/journal.pbio.1001874. View

11.

Barile L, Vassalli G . Exosomes: Therapy delivery tools and biomarkers of diseases. Pharmacol Ther. 2017; 174:63-78. DOI: 10.1016/j.pharmthera.2017.02.020. View

12.

Bebelman M, Smit M, Pegtel D, Baglio S . Biogenesis and function of extracellular vesicles in cancer. Pharmacol Ther. 2018; 188:1-11. DOI: 10.1016/j.pharmthera.2018.02.013. View

13.

Koynova R, Tenchov B, MacDonald R . Nonlamellar Phases in Cationic Phospholipids, Relevance to Drug and Gene Delivery. ACS Biomater Sci Eng. 2021; 1(3):130-138. DOI: 10.1021/ab500142w. View

14.

Zhang M, Hu S, Liu L, Dang P, Liu Y, Sun Z . Engineered exosomes from different sources for cancer-targeted therapy. Signal Transduct Target Ther. 2023; 8(1):124. PMC: 10017761. DOI: 10.1038/s41392-023-01382-y. View

15.

Hong M, Clubb J, Chen Y . Engineering CAR-T Cells for Next-Generation Cancer Therapy. Cancer Cell. 2020; 38(4):473-488. DOI: 10.1016/j.ccell.2020.07.005. View

16.

Huang H, Shao L, Chen Y, Tang L, Liu T, Li J . Synergistic strategy with hyperthermia therapy based immunotherapy and engineered exosomes-liposomes targeted chemotherapy prevents tumor recurrence and metastasis in advanced breast cancer. Bioeng Transl Med. 2022; 7(2):e10284. PMC: 9115690. DOI: 10.1002/btm2.10284. View

17.

Smyth T, Petrova K, Payton N, Persaud I, Redzic J, Graner M . Surface functionalization of exosomes using click chemistry. Bioconjug Chem. 2014; 25(10):1777-84. PMC: 4198107. DOI: 10.1021/bc500291r. View

18.

Cheng L, Zhang X, Tang J, Lv Q, Liu J . Gene-engineered exosomes-thermosensitive liposomes hybrid nanovesicles by the blockade of CD47 signal for combined photothermal therapy and cancer immunotherapy. Biomaterials. 2021; 275:120964. DOI: 10.1016/j.biomaterials.2021.120964. View

19.

Davies R, Kim J, Jang S, Choi E, Gho Y, Park J . Microfluidic filtration system to isolate extracellular vesicles from blood. Lab Chip. 2012; 12(24):5202-10. DOI: 10.1039/c2lc41006k. View

20.

Tenchov R, Sasso J, Wang X, Liaw W, Chen C, Zhou Q . Exosomes─Nature's Lipid Nanoparticles, a Rising Star in Drug Delivery and Diagnostics. ACS Nano. 2022; 16(11):17802-17846. PMC: 9706680. DOI: 10.1021/acsnano.2c08774. View