Molecular Prevalence and Associated Risk Factors of Spp. Infection in Dairy Cattle in Khon Kaen, Thailand

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2024 Apr 10

PMID 38595654

Authors

Bamphen Keomoungkhoun

I Putu Gede Yudhi Arjentinia

Somboon Sangmaneedet

Weerapol Taweenan

Affiliations

Soon will be listed here.

Abstract

Background And Aim: spp. are important parasites in the small intestines of humans and animals, particularly cattle. The aim of this study was to estimate the molecular prevalence and associated risk factors of infection in dairy cattle in five districts of Khon Kaen province, Thailand, and to identify spp.

Materials And Methods: From July 2020 to October 2021, 296 fecal samples were collected from three groups of dairy cattle: Calves aged <3 months, calves aged 3 months-1 year, and calves aged >1 year. spp. were detected by polymerase chain reaction (PCR) amplifying the 18s RNA gene. Both genus-specific and species-specific primers were used to identify confirmed by DNA sequencing. Age, house floor type, and water trough type were evaluated as risk factors. We analyzed all associated risk factor information using the logistic regression test in the Statistical Package for the Social Sciences.

Results: PCR results showed that 40 (13.51%) out of 296 samples were positive for spp., including (57.50%) and (2.50%). There was a significant association between incidence, cattle age, and house floor type (p < 0.05). National Center for Biotechnology Information Basic Local Alignment Search Tool displayed 99.48%-100% nucleotide similarity of each spp. isolate with references recorded on GenBank.

Conclusion: and are commonly found in dairy cattle, especially calves, in Khon Kaen, Thailand, and the incidence was associated with age and house floor type. A molecular technique may be influential for species identification. The results of the present study would provide useful information for veterinarians and animal owners to understand better spp. and how to manage farms properly.

Citing Articles

spp. Infection and Genotype Identification in Pre-Weaned and Post-Weaned Calves in Yunnan Province, China.

Deng M, Heng Z, Li L, Yang J, He J, Zou F Animals (Basel). 2024; 14(13).

PMID: 38998019 PMC: 11240314. DOI: 10.3390/ani14131907.

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