Integrated Transcriptome and Proteome Analysis Reveals That Cell Wall Activity Affects Parasitism

Overview

Journal Plants (Basel)

Date 2024 Apr 9

PMID 38592861

Authors

Meixiu Chen

Lu Zhang

Zhaoqun Yao

Xiaolei Cao

Qianqian Ma

Siyu Chen

Xuekun Zhang

Sifeng Zhao

Affiliations

Soon will be listed here.

Abstract

can infect many crops, causing large agricultural production losses. It is important to study the parasitism mechanism of to control its harm. In this experiment, the HY13M and TE9M from Tacheng Prefecture and Hami City in Xinjiang, respectively, were used to analyze the parasitical mechanism of by means of transcriptome and proteome analyses. The parasitic capacity of TE9M was significantly stronger than that of HY13M in . The results showed that the DEGs and DEPs were prominently enriched in the cell wall metabolism pathways, including "cell wall organization or biogenesis", "cell wall organization", and "cell wall". Moreover, the functions of the pectinesterase enzyme gene (), which is involved in the cell wall metabolism of in its parasitism, were studied by means HIGS. The number and weight of were significantly reduced when , which encodes a cell-wall-degrading protease, was silenced, indicating that it positively regulates parasitism. Thus, these results suggest that the cell wall metabolism pathway is involved in differentiation of the parasitic ability and that the gene plays an important role in 's parasitism.

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