Transcriptome Analysis of Reveals the Molecular Mechanisms of Ca Signaling Pathway on Arsenic Tolerance Induced by Arbuscular Mycorrhizal Fungi

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Apr 9

PMID 38591035

Authors

Minggui Gong

Na Bai

Jiajie Su

Yuan Wang

Yanan Wei

Qiaoming Zhang

Affiliations

Soon will be listed here.

Abstract

Introduction: Arbuscular mycorrhizal fungi (AMF) have been demonstrated their ability to enhance the arsenic (As) tolerance of host plants, and making the utilization of mycorrhizal plants a promising and practical approach for remediating As-contaminated soils. However, comprehensive transcriptome analysis to reveal the molecular mechanism of As tolerance in the symbiotic process between AMF and host plants is still limited.

Methods: In this study, transcriptomic analysis of seedlings was conducted with four treatments: non-inoculated under non-As stress (CK0), non-inoculated under As stress (CK100), -inoculated under non-As stress (FM0), and -inoculated under As stress (FM100).

Results: Our results showed that inoculation with led to a reduction in net fluxes of Ca, while increasing Ca contents in the roots and leaves of under the same As level in soil. Notably, 199 and 3129 differentially expressed genes (DEGs) were specially regulated by inoculation under As stress and non-As stress, respectively. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation and enrichment analyses, we found that under As stress, inoculation up-regulated a significant number of genes related to the Ca signaling pathway genes, involved in cellular process, membrane part, and signal transduction. This suggests a potential role in mitigating As tolerance in seedlings. Furthermore, our analysis identified specific DEGs in transcription factor families, including ERF, MYB, NAC, and WRKY, that were upregulated by inoculation. Conversely, MYB and HB-other were down-regulated. The ERF and MYB families exhibited the highest number of up- and down-regulated DEGs, respectively, which were speculated to play an important role in alleviating the As toxicity of .

Discussion: Our findings provided valuable insights into the molecular theoretical basis of the Ca signaling pathway in improving As tolerance of mycorrhizal plants in the future.

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