Rapid Generation of Fragrant Thermo-sensitive Genic Male Sterile Rice with Enhanced Disease Resistance Via CRISPR/Cas9

Overview

Journal Planta

Specialty Biology

Date 2024 Apr 6

PMID 38581602

Authors

Huali Zhang

Minmin Liang

Junyu Chen

Huimei Wang

Liangyong Ma

Affiliations

Soon will be listed here.

Abstract

The three, by mutagenesis produced genes OsPi21, OsXa5, and OsBADH, generated novel lines exhibiting desired fragrance and improved resistance. Elite sterile lines are the basis for hybrid rice breeding, and rice quality and disease resistance become the focus of new sterile lines breeding. Since there are few sterile lines with fragrance and high resistance to blast and bacterial blight at the same time in hybrid rice production, we here integrated the simultaneous mutagenesis of three genes, OsPi21, OsXa5, and OsBADH, into Zhi 5012S, an elite thermo-sensitive genic male sterile (TGMS) variety, using the CRISPR/Cas9 system, thus eventually generated novel sterile lines would exhibit desired popcorn-like fragrance and improved resistance to blast and bacterial blight but without a loss in major agricultural traits such as yield. Collectively, this study develops valuable germplasm resources for the development of two-line hybrid rice with disease resistance, which provides a way to rapid generation of novel TGMS lines with elite traits.

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