Brain Organoid Protocols and Limitations

Overview

Journal Front Cell Neurosci

Specialty Cell Biology

Date 2024 Apr 4

PMID 38572070

Authors

Helen H Zhao

Gabriel Haddad

Affiliations

Soon will be listed here.

Abstract

Stem cell-derived organoid technology is a powerful tool that revolutionizes the field of biomedical research and extends the scope of our understanding of human biology and diseases. Brain organoids especially open an opportunity for human brain research and modeling many human neurological diseases, which have lagged due to the inaccessibility of human brain samples and lack of similarity with other animal models. Brain organoids can be generated through various protocols and mimic whole brain or region-specific. To provide an overview of brain organoid technology, we summarize currently available protocols and list several factors to consider before choosing protocols. We also outline the limitations of current protocols and challenges that need to be solved in future investigation of brain development and pathobiology.

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