Screening, Identification, and Mechanism Analysis of Starch-degrading Bacteria During Curing Process in Tobacco Leaf

Overview

Journal Front Bioeng Biotechnol

Date 2024 Apr 3

PMID 38567082

Authors

Yan Zhang

Chuandong Jiang

Yangyang Li

Jingguo Sun

Zhenguo Chen

Qiang Zhang

Guangwei Sun

Affiliations

Soon will be listed here.

Abstract

Tobacco, a vital economic crop, had its quality post-curing significantly influenced by starch content. Nonetheless, the existing process parameters during curing were inadequate to satisfy the starch degradation requirements. Microorganisms exhibit inherent advantages in starch degradation, offering significant potential in the tobacco curing process. Our study concentrated on the microbial populations on the surface of tobacco leaves and in the rhizosphere soil. A strain capable of starch degradation, designated as BS3, was successfully isolated and identified as by phylogenetic tree analysis based on 16SrDNA sequence. The application of BS3 on tobacco significantly enhanced enzyme activity and accelerated starch degradation during the curing process. Furthermore, analyses of the metagenome, transcriptome, and metabolome indicated that the BS3 strain facilitated starch degradation by regulating surface microbiota composition and affecting genes related to starch hydrolyzed protein and key metabolites in tobacco leaves. This study offered new strategies for efficiently improving the quality of tobacco leaves.

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