The Neuroprotective Effect of Human Primary Astrocytes in Multiple Sclerosis: In Vitro Model

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2024 Apr 2

PMID 38564643

Authors

Amer Imraish

Tuqa Abu Thiab

Mohammad Alsalem

Saeed Dahbour

Hiba Khleif

Bashaer Abu-Irmaileh

Raneen Qasem

Khalid El-Salem

Affiliations

Soon will be listed here.

Abstract

Recent studies highlighted the role of astrocytes in neuroinflammatory diseases, particularly multiple sclerosis, interacting closely with other CNS components but also with the immune cells. However, due to the difficulty in obtaining human astrocytes, their role in these pathologies is still unclear. In this study we develop an astrocyte in vitro model to evaluate their role in multiple sclerosis after being treated with CSF isolated from both healthy and MS diagnosed patients. Gene expression and ELISA assays reveal that several pro-inflammatory markers IL-1β, TNF-α and IL-6, were significantly downregulated in astrocytes treated with MS-CSF. In contrast, neurotrophic survival, and growth factors, and GFAP, BDNF, GDNF and VEGF, were markedly elevated upon the same treatment. In summary, this study supports the notion of the astrocyte involvement in MS. The results reveal the neuroprotective role of astrocyte in MS pathogenicity by suppressing excessive inflammation and increasing the expression of tropic factors.

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