Molecular Characterization and Prevalence of β-lactamase-producing Enterobacterales in Livestock and Poultry Slaughterhouses Wastewater in Iran

Overview

Journal J Water Health

Specialties Environmental Health
Public Health

Date 2024 Apr 1

PMID 38557572

Authors

Mehran Sardari

Meysam Manouchehrifar

Kamal Hasani

Nasrin Habibzadeh

Hadi Peeri Doghaheh

Taher Azimi

Mohsen Arzanlou

Affiliations

Soon will be listed here.

Abstract

Beta-lactamase-producing Enterobacterales bacteria cause severe hard-to-treat infections. Currently, they are spreading beyond hospitals and becoming a serious global health concern. This study investigated the prevalence and molecular characterization of extended-spectrum β-lactamase and AmpC-type β-lactamase-producing Enterobacterales (ESBL-PE, AmpC-PE) in wastewater from livestock and poultry slaughterhouses in Ardabil, Iran. A total of 80 Enterobacterales bacteria belonging to 9 species were identified. Among the isolates, Escherichia coli (n = 21/80; 26.2%) and Citrobacter spp. (n = 18/80; 22.5%) exhibited the highest frequency. Overall, 18.7% (n = 15/80) and 2.5% (n = 2/80) of Enterobacterales were found to be ESBL and AmpC producers, respectively. The most common ESBL producer isolates were E. coli (n = 9/21; 42.8%) and Klebsiella pneumoniae (n = 6/7; 85.7%). All AmpC-PE isolates belonged to E. coli strains (n = 2/21; 9.5%). In this study, 80% of ESBL-PE and 100% of AmpC-PE isolates were recovered from poultry slaughterhouse wastewater. All ESBL-PE and AmpC-PE isolates were multidrug-resistant. In total, 93.3% of ESBL-PE isolates harbored the bla gene, with the bla being the most common subgroup. The emergence of ESBL-PE and AmpC-PE in wastewater of food-producing animals allows for zoonotic transmission to humans through contaminated food products and contaminations of the environment.

Citing Articles

Carbapenem-resistant in wastewater resources and healthy carriers: A survey in Iran.

Khavandi S, Habibzadeh N, Hasani K, Sardari M, Arzanlou M J Water Health. 2024; 22(6):1053-1063.

PMID: 38935456 DOI: 10.2166/wh.2024.041.

Exploring Extended-Spectrum Beta-Lactamase (ESBL)-Producing in Food-Producing Animals and Animal-Derived Foods.

Ribeiro L, Nespolo N, Rossi G, Fairbrother J Pathogens. 2024; 13(4).

PMID: 38668301 PMC: 11054374. DOI: 10.3390/pathogens13040346.

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