Single-cell Adhesive Profiling in an Optofluidic Device Elucidates CD8 T Lymphocyte Phenotypes in Inflamed Vasculature-like Microenvironments

Overview

Journal Cell Rep Methods

Specialty Cell Biology

Date 2024 Mar 30

PMID 38554703

Authors

Camila P Camargo

Yunus Alapan

Abir K Muhuri

Samuel N Lucas

Susan N Thomas

Affiliations

Soon will be listed here.

Abstract

Tissue infiltration by circulating leukocytes occurs via adhesive interactions with the local vasculature, but how the adhesive quality of circulating cells guides the homing of specific phenotypes to different vascular microenvironments remains undefined. We developed an optofluidic system enabling fluorescent labeling of photoactivatable cells based on their adhesive rolling velocity in an inflamed vasculature-mimicking microfluidic device under physiological fluid flow. In so doing, single-cell level multidimensional profiling of cellular characteristics could be characterized and related to the associated adhesive phenotype. When applied to CD8 T cells, ligand/receptor expression profiles and subtypes associated with adhesion were revealed, providing insight into inflamed tissue infiltration capabilities of specific CD8 T lymphocyte subsets and how local vascular microenvironmental features may regulate the quality of cellular infiltration. This methodology facilitates rapid screening of cell populations for enhanced homing capabilities under defined biochemical and biophysical microenvironments, relevant to leukocyte homing modulation in multiple pathologies.

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