Investigating Vα7.2/CD161 T Cell and MAIT Cell Profiles Using Flow Cytometry in Healthy Subjects and Subjects with Atopic Dermatitis

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Mar 28

PMID 38542456

Authors

Parvind Singh

Krisztian Gaspar

Andrea Szegedi

Laszlo Sajtos

Sandor Barath

Zsuzsanna Hevessy

Affiliations

Soon will be listed here.

Abstract

This study investigates the roles of mucosal-associated invariant T (MAIT) cells and Vα7.2/CD161 T cells in skin diseases, focusing on atopic dermatitis. MAIT cells, crucial for bridging innate and adaptive immunity, were analyzed alongside Vα7.2/CD161 T cells in peripheral blood samples from 14 atopic dermatitis patients and 10 healthy controls. Flow cytometry and machine learning algorithms were employed for a comprehensive analysis. The results indicate a significant decrease in MAIT cells and CD69 subsets in atopic dermatitis, coupled with elevated CD38 and polyfunctional MAIT cells producing TNFα and Granzyme B (TNFα/GzB). Vα7.2/CD161 T cells in atopic dermatitis exhibited a decrease in CD8 and IFNγ-producing subsets but an increase in CD38 activated and IL-22-producing subsets. These results highlight the distinctive features of MAIT cells and Vα7.2/CD161 T cells and their different roles in the pathogenesis of atopic dermatitis and provide insights into their potential roles in immune-mediated skin diseases.

Citing Articles

A Comprehensive Investigation of Stimulatory Agents on MAIT and Vα7.2+/CD161- T Cell Response and Effects of Immunomodulatory Drugs.

Singh P, Szaraz-Szeles M, Barath S, Hevessy Z Int J Mol Sci. 2024; 25(11).

PMID: 38892082 PMC: 11172258. DOI: 10.3390/ijms25115895.

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