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Genome Constitution and Evolution of Elymus Atratus (Poaceae: Triticeae) Inferred from Cytogenetic and Phylogenetic Analysis

Overview
Journal Genes Genomics
Specialty Genetics
Date 2024 Mar 27
PMID 38536618
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Abstract

Background: Elymus atratus (Nevski) Hand.-Mazz. is perennial hexaploid wheatgrass. It was assigned to the genus Elymus L. sensu stricto based on morphological characters. Its genome constitution has not been disentangled yet.

Objective: To identify the genome constitution and origin of E. atratus.

Methods: In this study, genomic in situ hybridization and fluorescence in situ hybridization, and phylogenetic analysis based on the Acc1, DMC1 and matK sequences were performed.

Results: Genomic in situ hybridization and fluorescence in situ hybridization results reveal that E. atratus 2n = 6x = 42 is composed of 14 St genome chromosomes, 14 H genome chromosomes, and 14 Y genome chromosomes including two H-Y type translocation chromosomes, suggesting that the genome formula of E. atratus is StStYYHH. The phylogenetic analysis based on Acc1 and DMC1 sequences not only shows that the Y genome originated in a separate diploid, but also suggests that Pseudoroegneria (St), Hordeum (H), and a diploid species with Y genome were the potential donors of E. atratus. Data from chloroplast DNA showed that the maternal donor of E. atratus contains the St genome.

Conclusion: Elymus atratus is an allohexaploid species with StYH genome, which may have originated through the hybridization between an allotetraploid Roegneria (StY) species as the maternal donor and a diploid Hordeum (H) species as the paternal donor.

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References
1.
Adderley S, Sun G . Molecular evolution and nucleotide diversity of nuclear plastid phosphoglycerate kinase (PGK) gene in Triticeae (Poaceae). Gene. 2013; 533(1):142-8. DOI: 10.1016/j.gene.2013.09.103. View

2.
Dong Z, Fan X, Sha L, Wang Y, Zeng J, Kang H . Phylogeny and differentiation of the St genome in Elymus L. sensu lato (Triticeae; Poaceae) based on one nuclear DNA and two chloroplast genes. BMC Plant Biol. 2015; 15:179. PMC: 4499217. DOI: 10.1186/s12870-015-0517-2. View

3.
Guindon S, Delsuc F, Dufayard J, Gascuel O . Estimating maximum likelihood phylogenies with PhyML. Methods Mol Biol. 2009; 537:113-37. DOI: 10.1007/978-1-59745-251-9_6. View

4.
Han F, Gao Z, Birchler J . Reactivation of an inactive centromere reveals epigenetic and structural components for centromere specification in maize. Plant Cell. 2009; 21(7):1929-39. PMC: 2729603. DOI: 10.1105/tpc.109.066662. View

5.
Huang S, Sirikhachornkit A, Su X, Faris J, Gill B, Haselkorn R . Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat. Proc Natl Acad Sci U S A. 2002; 99(12):8133-8. PMC: 123033. DOI: 10.1073/pnas.072223799. View