Construction of a Cosmid-Based Ultraefficient Genomic Library System for Filamentous Fungi of the Genus

Overview

Journal J Fungi (Basel)

Date 2024 Mar 27

PMID 38535197

Authors

Chihiro Kadooka

Takuji Oka

Affiliations

Soon will be listed here.

Abstract

Filamentous fungi of the genus include producers of industrially important organic acids, enzymes, and secondary metabolites, as well as pathogens of many plants and animals. Novel genes in the genome are potentially crucial for the fermentation and drug industries (e.g., agrochemicals and antifungal drugs). A research approach based on classical genetics is effective for identifying functionally unknown genes. During analyses based on classical genetics, mutations must be identified easily and quickly. Herein, we report the development of a cosmid-based plasmid pTOCK1 and the use of a genomic library of constructed using pTOCK1. The cosmid-based genomic library was used for convenient auxotrophic mutants ( and ), as well as mutants with abnormal colony morphology () and yellow conidia (), to obtain library clones complementary to these phenotypes. The complementary strain could be obtained through a single transformation, and the cosmid could be rescued. Thus, our cosmid library system can be used to identify the causative gene in a mutant strain.

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