Vacuum Plasma Treatment Device for Enhancing Fibroblast Activity on Machined and Rough Titanium Surfaces

Overview

Journal Dent J (Basel)

Specialty Dentistry

Date 2024 Mar 27

PMID 38534295

Authors

Luigi Canullo

Tullio Genova

Giorgia Chinigo

Roberta Iacono

Paolo Pesce

Maria Menini

Federico Mussano

Affiliations

Soon will be listed here.

Abstract

This study was conducted to compare the effects of an innovative plasma surface treatment device that does not need a gas supply for titanium disks with two different surface topographies: the prototypical machined surface (MAC) and one of the most diffused roughened ones (SL) obtained through grit blasting and acid etching. A total of 200-MAC and 200-SL titanium disks were used. Each group of disks was divided into four sub-groups of 40 samples each that were subjected to five different tests. Among these, 150-MAC and 150-SL were considered the test group, and they were treated with plasma for 15, 30, and 60 s after being removed from the sterile packaging. On the other hand, 50-MAC and 50-SL were considered the control group, and they were only removed from sterile plastic vials. The samples were analyzed to evaluate the capability of the plasma treatment in influencing protein adsorption, cell adhesion, proliferation, and microbial growth on the test group disks when compared to the untreated disks. Protein adsorption was significantly enhanced after 20 min of plasma treatment for 15 and 30 s on the MAC and SL disks. Plasma treatment for 15 and 30 s significantly increased the level of adhesion in both treated samples after 30 min. Furthermore, the MAC samples showed a significant increase in cell adhesion 4 h after plasma treatment for 15 s. The SEM analysis highlighted that, on the treated samples (especially on the MAC disks), the cells with a polygonal and flat shape prevailed, while the fusiform- and globular-shaped cells were rare. The encouraging results obtained further confirm the effectiveness of plasma treatments on cell adhesion and fibroblast activity.

Citing Articles

Comparative Investigation of Vortex and Direct Plasma Discharge for Treating Titanium Surface.

Jeon H, Seo S, Jung A, Kang K, Lee J, Gweon B Biomimetics (Basel). 2025; 10(1).

PMID: 39851723 PMC: 11759839. DOI: 10.3390/biomimetics10010007.

References

Mehl C, Kern M, Zimmermann A, Harder S, Huth S, Selhuber-Unkel C . Impact of Cleaning Procedures on Adhesion of Living Cells to Three Abutment Materials. Int J Oral Maxillofac Implants. 2017; 32(5):976-984. DOI: 10.11607/jomi.5630. View

Garcia B, Camacho F, Penarrocha D, Tallarico M, Perez S, Canullo L . Influence of plasma cleaning procedure on the interaction between soft tissue and abutments: a randomized controlled histologic study. Clin Oral Implants Res. 2016; 28(10):1269-1277. DOI: 10.1111/clr.12953. View

Mishra P, Wu W, Rozo C, Hallab N, Benevenia J, Gause W . Micrometer-sized titanium particles can induce potent Th2-type responses through TLR4-independent pathways. J Immunol. 2011; 187(12):6491-8. PMC: 3237784. DOI: 10.4049/jimmunol.1101392. View

Lambrechts T, Doornewaard R, De Bruyckere T, Matthijs L, Deschepper E, Cosyn J . A multicenter cohort study on the association of the one-abutment one-time concept with marginal bone loss around bone level implants. Clin Oral Implants Res. 2020; 32(2):192-202. DOI: 10.1111/clr.13689. View

Sculean A, Gruber R, Bosshardt D . Soft tissue wound healing around teeth and dental implants. J Clin Periodontol. 2014; 41 Suppl 15:S6-22. DOI: 10.1111/jcpe.12206. View

Hamudi N, Barnea E, Weinberg E, Laviv A, Mijiritsky E, Matalon S . The Association of the One-Abutment at One-Time Concept with Marginal Bone Loss around the SLA and Platform Switch and Conical Abutment Implants. J Clin Med. 2022; 11(1). PMC: 8745541. DOI: 10.3390/jcm11010074. View

Canullo L, Penarrocha Oltra D, Pesce P, Zarauz C, Lattanzio R, Penarrocha Diago M . Soft tissue integration of different abutment surfaces: An experimental study with histological analysis. Clin Oral Implants Res. 2021; 32(8):928-940. DOI: 10.1111/clr.13782. View

Molina A, Sanz-Sanchez I, Martin C, Blanco J, Sanz M . The effect of one-time abutment placement on interproximal bone levels and peri-implant soft tissues: a prospective randomized clinical trial. Clin Oral Implants Res. 2016; 28(4):443-452. DOI: 10.1111/clr.12818. View

Sabetrasekh R, Tiainen H, Reseland J, Will J, Ellingsen J, Lyngstadaas S . Impact of trace elements on biocompatibility of titanium scaffolds. Biomed Mater. 2010; 5(1):15003. DOI: 10.1088/1748-6041/5/1/015003. View

10.

Nevins M, Chen C, Parma-Benfenati S, Kim D . Gas Plasma Treatment Improves Titanium Dental Implant Osseointegration-A Preclinical In Vivo Experimental Study. Bioengineering (Basel). 2023; 10(10). PMC: 10604242. DOI: 10.3390/bioengineering10101181. View

11.

Blazquez-Hinarejos M, Ayuso-Montero R, Jane-Salas E, Lopez-Lopez J . Influence of surface modified dental implant abutments on connective tissue attachment: A systematic review. Arch Oral Biol. 2017; 80:185-192. DOI: 10.1016/j.archoralbio.2017.04.020. View

12.

Souza J, Sordi M, Kanazawa M, Ravindran S, Henriques B, Silva F . Nano-scale modification of titanium implant surfaces to enhance osseointegration. Acta Biomater. 2019; 94:112-131. DOI: 10.1016/j.actbio.2019.05.045. View

13.

Abrahamsson I, Berglundh T, Lindhe J . Soft tissue response to plaque formation at different implant systems. A comparative study in the dog. Clin Oral Implants Res. 1998; 9(2):73-9. DOI: 10.1034/j.1600-0501.1998.090202.x. View

14.

Hermann J, Cochran D, Nummikoski P, Buser D . Crestal bone changes around titanium implants. A radiographic evaluation of unloaded nonsubmerged and submerged implants in the canine mandible. J Periodontol. 1998; 68(11):1117-30. DOI: 10.1902/jop.1997.68.11.1117. View

15.

Vezeau P, Koorbusch G, Draughn R, Keller J . Effects of multiple sterilization on surface characteristics and in vitro biologic responses to titanium. J Oral Maxillofac Surg. 1996; 54(6):738-46. DOI: 10.1016/s0278-2391(96)90694-1. View

16.

Gehrke P, Smeets R, Gosau M, Friedrich R, Madani E, Duddeck D . The Influence of an Ultrasonic Cleaning Protocol for CAD/CAM Abutment Surfaces on Cell Viability and Inflammatory Response . In Vivo. 2019; 33(3):689-698. PMC: 6559919. DOI: 10.21873/invivo.11527. View

17.

Lee H, Jeon H, Jung A, Kim J, Kim J, Lee S . Improvement of osseointegration efficacy of titanium implant through plasma surface treatment. Biomed Eng Lett. 2022; 12(4):421-432. PMC: 9551159. DOI: 10.1007/s13534-022-00245-9. View

18.

Canullo L, Cassinelli C, Gotz W, Tarnow D . Plasma of argon accelerates murine fibroblast adhesion in early stages of titanium disk colonization. Int J Oral Maxillofac Implants. 2013; 28(4):957-62. DOI: 10.11607/jomi.2664. View

19.

Pommer B, Danzinger M, Leite Aiquel L, Pitta J, Haas R . Long-term outcomes of maxillary single-tooth implants in relation to timing protocols of implant placement and loading: Systematic review and meta-analysis. Clin Oral Implants Res. 2021; 32 Suppl 21:56-66. DOI: 10.1111/clr.13838. View

20.

Kim S, Choi C, Cha Y, Chang J . The efficacy of convenient cleaning methods applicable for customized abutments: an in vitro study. BMC Oral Health. 2021; 21(1):78. PMC: 7890105. DOI: 10.1186/s12903-021-01436-z. View