Reduced Cardiac Antioxidant Defenses Mediate Increased Susceptibility to Workload-induced Myocardial Injury in Males with Genetic Cardiomyopathy

Overview

Journal J Mol Cell Cardiol

Specialty Cardiology & Vascular Diseases

Date 2024 Mar 25

PMID 38527667

Authors

Tatyana A Vetter

Preethy Parthiban

Jackie A Stevens

Xavier S Revelo

Mark J Kohr

DeWayne Townsend

Affiliations

Soon will be listed here.

Abstract

Ongoing cardiomyocyte injury is a major mechanism in the progression of heart failure, particularly in dystrophic hearts. Due to the poor regenerative capacity of the adult heart, cardiomyocyte death results in the permanent loss of functional myocardium. Understanding the factors contributing to myocyte injury is essential for the development of effective heart failure therapies. As a model of persistent cardiac injury, we examined mice lacking β-sarcoglycan (β-SG), a key component of the dystrophin glycoprotein complex (DGC). The loss of the sarcoglycan complex markedly compromises sarcolemmal integrity in this β-SG model. Our studies aim to characterize the mechanisms underlying dramatic sex differences in susceptibility to cardiac injury in β-SG mice. Male β-SG hearts display significantly greater myocardial injury and death following isoproterenol-induced cardiac stress than female β-SG hearts. This protection of females was independent of ovarian hormones. Male β-SG hearts displayed increased susceptibility to exogenous oxidative stress and were significantly protected by angiotensin II type 1 receptor (ATR) antagonism. Increasing general antioxidative defenses or increasing the levels of S-nitrosylation both provided protection to the hearts of β-SG male mice. Here we demonstrate that increased susceptibility to oxidative damage leads to an ATR-mediated amplification of workload-induced myocardial injury in male β-SG mice. Improving oxidative defenses, specifically by increasing S-nitrosylation, provided protection to the male β-SG heart from workload-induced injury. These studies describe a unique susceptibility of the male heart to injury and may contribute to the sex differences in other forms of cardiac injury.

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