High Biocompatible FITC-conjugated Silica Nanoparticles for Cell Labeling in Both in Vitro and in Vivo Models

Overview

Journal Sci Rep

Specialty Science

Date 2024 Mar 24

PMID 38521815

Authors

Thi Thuy Nguyen

Hoang Nam Nguyen

Thi Ha Lien Nghiem

Xuan-Hai Do

Thanh Thuy To

Thi Xuan Phuong Do

Dieu Linh Do

Huong Giang Nguyen

Huy Manh Nguyen

Ngoc Dinh Nguyen

Manh Quynh Luu

Trong Nghia Nguyen

Thi Bich Ngoc Nguyen

Van Toan Nguyen

Van Thanh Pham

Uyen Thi Trang Than

Thi My Nhung Hoang

Affiliations

Soon will be listed here.

Abstract

Fluorescence nanosilica-based cell tracker has been explored and applied in cell biological research. However, the aggregation of these nanoparticles at physiological pH is still the main limitation. In this research, we introduced a novel fluorescence nano-based cell tracker suitable for application in live cells. The silica-coated fluorescein isothiocyanate isomer (FITC-SiO) nanoparticles (NPs) were modified with carboxymethylsilanetriol disodium salt (FITC-SiO-COOH), integrating the dianion form of FITC molecules. This nanosystem exhibited superior dispersion in aqueous solutions and effectively mitigated dye leakage. These labeled NPs displayed notable biocompatibility and minimal cytotoxicity in both in vitro and in vivo conditions. Significantly, the NPs did not have negative implications on cell migration or angiogenesis. They successfully penetrated primary fibroblasts, human umbilical vein endothelial cells and HeLa cells in both 2D and 3D cultures, with the fluorescence signal enduring for over 72 h. Furthermore, the NP signals were consistently observed in the developing gastrointestinal tract of live medaka fish larvae for extended periods during phases of subdued digestive activity, without manifesting any apparent acute toxicity. These results underscore the promising utility of FITC-SiO-COOH NPs as advanced live cell trackers in biological research.

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