The Micro CT Evaluation of Crown and Root Pulp Volume Versus Dentin Thickness in Teeth in Postmortem Interval (PMI)

Overview

Journal Forensic Sci Med Pathol

Specialties Forensic Sciences
Pathology

Date 2024 Mar 21

PMID 38512597

Authors

Selcuk Cetin

Nihat Akbulut

Kaan Orhan

Burak Bilecenoglu

Mert Ocak

Emre Bayram

Ahmet Altan

Bulent Eren

Serkan Silsupur

Bedirhan Sezer Oner

Affiliations

Soon will be listed here.

Abstract

Determining the postmortem interval (PMI) is one of the main study subjects of forensic sciences. The main purpose of this prospective in vitro study that was the Micro-CT evaluation of teeth crown and root pulp volume versus dentin thickness in terms of PMI determination. The study involved 60 female Wistar rats, with weights ranging from 270 to 320 g. These rats were grouped into six different post-mortem period categories. Following the animals' sacrifice, they were subjected to a natural putrefaction period, with a control group, in the grounds of a sheltered garden. Hemi-mandible samples were then extracted and placed in glass tubes for Micro-CT evaluations, following the progression of putrefaction processes. The pulp volume and dentin thickness were assessed using Micro-CT, and the gathered data underwent statistical analysis. Micro-CT was employed to analyze sixty right mandibular second molar teeth in the hemi-mandible. The crown pulp volume exhibited a reduction in group 6, with a value of 0.239 mm after a three-month period of natural putrefaction (p < 0.001). There is statistically differences among groups in case of pairwise comparison (p < 0.05). However, the root pulp volume and dentin thickness variables did not display any statistically significant changes. Despite certain limitations associated with this study, the Micro-CT findings concerning teeth pulp volume can serve as an objective parameter, especially for late postmortem investigations and the estimation of time of death.

References

Vavpotic M, Turk T, Martincic D, Balazic J . Characteristics of the number of odontoblasts in human dental pulp post-mortem. Forensic Sci Int. 2009; 193(1-3):122-6. DOI: 10.1016/j.forsciint.2009.09.023. View

Bose C, Kshirsagar S, Vijayan M, Kumar S, Singh S, Hindle A . The role of RLIP76 in oxidative stress and mitochondrial dysfunction: Evidence based on autopsy brains from Alzheimer's disease patients. Biochim Biophys Acta Mol Basis Dis. 2023; 1870(2):166932. DOI: 10.1016/j.bbadis.2023.166932. View

Xiang Q, Chen L, Su Q, Du Y, Liang P, Kang X . Research Progress on Microbial Community Succession in the Postmortem Interval Estimation. Fa Yi Xue Za Zhi. 2023; 39(4):399-405. DOI: 10.12116/j.issn.1004-5619.2022.420606. View

Moitas B, Caldas I, Sampaio-Maia B . Microbiology and postmortem interval: a systematic review. Forensic Sci Med Pathol. 2023; 20(2):696-715. PMC: 11297127. DOI: 10.1007/s12024-023-00733-z. View

Roy J, Shahu U, Shirpure P, Soni S, Parekh U, Johnson A . A literature review on dental autopsy - an invaluable investigative technique in forensics. Autops Case Rep. 2021; 11:e2021295. PMC: 8387071. DOI: 10.4322/acr.2021.295. View

Demiralp K, Kursun Cakmak S, Aksoy S, Bayrak S, Orhan K, Demir P . Assessment of paranasal sinus parameters according to ancient skulls' gender and age by using cone-beam computed tomography. Folia Morphol (Warsz). 2018; 78(2):344-350. DOI: 10.5603/FM.a2018.0089. View

Wenzlow N, Mills D, Byrd J, Warren M, Long M . Review of the current and potential use of biological and molecular methods for the estimation of the postmortem interval in animals and humans. J Vet Diagn Invest. 2023; 35(2):97-108. PMC: 9999395. DOI: 10.1177/10406387231153930. View

Gemmellaro M, Hamilton G, Ware J . Review of Molecular Identification Techniques for Forensically Important Diptera. J Med Entomol. 2019; 56(4):887-902. DOI: 10.1093/jme/tjz040. View

Bakdash A, Kumar S, Gautam K, Mishra V . Use of flow cytometry in forensic medicine: Current scenario and future prospects. J Forensic Leg Med. 2018; 60:42-44. DOI: 10.1016/j.jflm.2018.09.010. View

10.

Woess C, Huck C, Badzoka J, Kappacher C, Arora R, Lindtner R . Raman spectroscopy for postmortem interval estimation of human skeletal remains: A scoping review. J Biophotonics. 2023; 16(10):e202300189. DOI: 10.1002/jbio.202300189. View

11.

Dawson B, Ueland M, Carter D, Mclntyre D, Barton P . Bridging the gap between decomposition theory and forensic research on postmortem interval. Int J Legal Med. 2023; 138(2):509-518. PMC: 10861637. DOI: 10.1007/s00414-023-03060-8. View

12.

Pittner S, Ehrenfellner B, Monticelli F, Zissler A, Sanger A, Stoiber W . Postmortem muscle protein degradation in humans as a tool for PMI delimitation. Int J Legal Med. 2016; 130(6):1547-1555. PMC: 5055573. DOI: 10.1007/s00414-016-1349-9. View

13.

Ducloyer M, David A, Dautreme B, Tournel G, Vincent F, Clement R . Pictorial review of the postmortem computed tomography in neonaticide cases. Int J Legal Med. 2021; 135(6):2395-2408. DOI: 10.1007/s00414-021-02677-x. View

14.

Ducloyer M, Tuchtan L, Delteil C, Piercecchi M, David A, Visseaux G . Lung density measurement in postmortem computed tomography: a new tool to assess immediate neonatal breath in suspected neonaticides. Int J Legal Med. 2019; 134(3):1159-1166. DOI: 10.1007/s00414-019-02103-3. View

15.

Srirangarajan S, Sindhu V, Raju S, Rao R, Prabhu S, Rudresh V . Evaluation of gingival tissue samples for predicting the time of death using histological and biochemical tests. Forensic Sci Int. 2021; 324:110850. DOI: 10.1016/j.forsciint.2021.110850. View

16.

Alibegovic A . Cartilage: a new parameter for the determination of the postmortem interval?. J Forensic Leg Med. 2014; 27:39-45. DOI: 10.1016/j.jflm.2014.08.005. View

17.

Higgins D, Austin J . Teeth as a source of DNA for forensic identification of human remains: a review. Sci Justice. 2013; 53(4):433-41. DOI: 10.1016/j.scijus.2013.06.001. View

18.

Sevgi U, Johnsen G, Hussain B, Piasecki L, Nogueira L, Haugen H . Morphometric micro-CT study of contralateral mandibular incisors. Clin Oral Investig. 2023; 28(1):20. PMC: 10751267. DOI: 10.1007/s00784-023-05419-y. View

19.

Monteiro J, Takusagawa T, Sampaio G, He H, de Oliveira E Silva E, Vasconcelos B . Gelatin methacryloyl hydrogel with and without dental pulp stem cells for TMJ regeneration: An in vivo study in rabbits. J Oral Rehabil. 2023; 51(2):394-403. DOI: 10.1111/joor.13608. View

20.

Jannati R, Afshari M, Moosazadeh M, Allahgholipour S, Eidy M, Hajihoseini M . Prevalence of pulp stones: A systematic review and meta-analysis. J Evid Based Med. 2018; 12(2):133-139. DOI: 10.1111/jebm.12331. View