Molecular Analysis of the Extracellular Microenvironment: from Form to Function

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2024 Mar 21

PMID 38509768

Authors

Jade K Macdonald

Anand S Mehta

Richard R Drake

Peggi M Angel

Affiliations

Soon will be listed here.

Abstract

The extracellular matrix (ECM) proteome represents an important component of the tissue microenvironment that controls chemical flux and induces cell signaling through encoded structure. The analysis of the ECM represents an analytical challenge through high levels of post-translational modifications, protease-resistant structures, and crosslinked, insoluble proteins. This review provides a comprehensive overview of the analytical challenges involved in addressing the complexities of spatially profiling the extracellular matrix proteome. A synopsis of the process of synthesizing the ECM structure, detailing inherent chemical complexity, is included to present the scope of the analytical challenge. Current chromatographic and spatial techniques addressing these challenges are detailed. Capabilities for multimodal multiplexing with cellular populations are discussed with a perspective on developing a holistic view of disease processes that includes both the cellular and extracellular microenvironment.

Citing Articles

The Spatial Extracellular Proteomic Tumor Microenvironment Distinguishes Molecular Subtypes of Hepatocellular Carcinoma.

Macdonald J, Taylor H, Wang M, DelaCourt A, Edge C, Lewin D J Proteome Res. 2024; 23(9):3791-3805.

PMID: 38980715 PMC: 11385377. DOI: 10.1021/acs.jproteome.4c00099.

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