Open-top Multisample Dual-view Light-sheet Microscope for Live Imaging of Large Multicellular Systems

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2024 Mar 21

PMID 38509326

Authors

Franziska Moos

Simon Suppinger

Gustavo de Medeiros

Koen Cornelius Oost

Andrea Boni

Camille Remy

Sera Lotte Weevers

Charisios Tsiairis

Petr Strnad

Prisca Liberali

Affiliations

Soon will be listed here.

Abstract

Multicellular systems grow over the course of weeks from single cells to tissues or even full organisms, making live imaging challenging. To bridge spatiotemporal scales, we present an open-top dual-view and dual-illumination light-sheet microscope dedicated to live imaging of large specimens at single-cell resolution. The configuration of objectives together with a customizable multiwell mounting system combines dual view with high-throughput multiposition imaging. We use this microscope to image a wide variety of samples and highlight its capabilities to gain quantitative single-cell information in large specimens such as mature intestinal organoids and gastruloids.

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