Histological Evaluation of the Alpaca (Vicugna Pacos) Vomeronasal Organ

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 2024 Mar 20

PMID 38508726

Authors

Sungmoo Hong

Meejung Ahn

Changjong Moon

Irene Ortiz-Leal

Pablo Sanchez-Quinteiro

Taeyoung Kang

Taekyun Shin

Affiliations

Soon will be listed here.

Abstract

Little is known about the neuronal structure of the vomeronasal organ (VNO), a receptor organ responsible for pheromone perception, in the alpaca (Vicugna pacos). This study was performed to determine the localization of neuronal elements, including protein gene product 9.5 (PGP 9.5), a pan-neuronal marker, olfactory marker protein (OMP), a marker of mature olfactory receptor cells, and phospholipase C beta 2 (PLC-β2), a marker of solitary chemoreceptor cells (SCCs), in the VNO. OMP was identified in receptor cells of the vomeronasal sensory epithelium (VSE), while PGP 9.5 and PLC-β2 were localized in both the VSE and vomeronasal non-sensory epithelium. Collectively, these results suggested that the alpaca VNO possesses SCCs and olfactory receptor cells, which recognize both harmful substances and pheromones.

References

Salazar I, Lombardero M, Aleman N, Sanchez Quinteiro P . Development of the vomeronasal receptor epithelium and the accessory olfactory bulb in sheep. Microsc Res Tech. 2003; 61(5):438-47. DOI: 10.1002/jemt.10362. View

Taniguchi K, Mikami S . Fine structure of the epithelia of the vomeronasal organ of horse and cattle. A comparative study. Cell Tissue Res. 1985; 240(1):41-8. DOI: 10.1007/BF00217556. View

Hong S, Weerasinghe-Mudiyanselage P, Kang S, Moon C, Shin T . Retinal transcriptome profiling identifies novel candidate genes associated with visual impairment in a mouse model of multiple sclerosis. Anim Cells Syst (Seoul). 2023; 27(1):219-233. PMC: 10552570. DOI: 10.1080/19768354.2023.2264354. View

Oikawa T, Saito H, Taniguchi K . Immunohistochemical studies on the differential maturation of three types of olfactory organs in the rats. J Vet Med Sci. 2001; 63(7):759-65. DOI: 10.1292/jvms.63.759. View

Takigami S, Mori Y, Ichikawa M . Projection pattern of vomeronasal neurons to the accessory olfactory bulb in goats. Chem Senses. 2000; 25(4):387-93. DOI: 10.1093/chemse/25.4.387. View

Yokosuka M . Histological properties of the glomerular layer in the mouse accessory olfactory bulb. Exp Anim. 2012; 61(1):13-24. DOI: 10.1538/expanim.61.13. View

Finger T, Bottger B, Hansen A, Anderson K, Alimohammadi H, Silver W . Solitary chemoreceptor cells in the nasal cavity serve as sentinels of respiration. Proc Natl Acad Sci U S A. 2003; 100(15):8981-6. PMC: 166424. DOI: 10.1073/pnas.1531172100. View

Kondoh D, Tomiyasu J, Itakura R, Sugahara M, Yanagawa M, Watanabe K . Comparative histological studies on properties of polysaccharides secreted by vomeronasal glands of eight Laurasiatheria species. Acta Histochem. 2020; 122(3):151515. DOI: 10.1016/j.acthis.2020.151515. View

Lee K, Park C, Kim J, Moon C, Ahn M, Shin T . Histological and lectin histochemical studies of the vomeronasal organ of horses. Tissue Cell. 2016; 48(4):361-9. DOI: 10.1016/j.tice.2016.05.002. View

10.

Ibrahim D . Immunolocalization of Receptor and Chemoreceptor Modules in the Sheep Vomeronasal Organ. Cells Tissues Organs. 2018; 205(2):85-92. DOI: 10.1159/000487758. View

11.

Kim B, Kim J, Youn B, Moon C . Dopamine depletion alters neuroplasticity-related signaling in the rat hippocampus. Anim Cells Syst (Seoul). 2023; 27(1):436-446. PMC: 10732217. DOI: 10.1080/19768354.2023.2294308. View

12.

Ogura T, Krosnowski K, Zhang L, Bekkerman M, Lin W . Chemoreception regulates chemical access to mouse vomeronasal organ: role of solitary chemosensory cells. PLoS One. 2010; 5(7):e11924. PMC: 2912856. DOI: 10.1371/journal.pone.0011924. View

13.

Zancanaro C, Caretta C, Merigo F, Cavaggioni A, Osculati F . alpha-Gustducin expression in the vomeronasal organ of the mouse. Eur J Neurosci. 1999; 11(12):4473-5. DOI: 10.1046/j.1460-9568.1999.00912.x. View

14.

Dibattista M, Al Koborssy D, Genovese F, Reisert J . The functional relevance of olfactory marker protein in the vertebrate olfactory system: a never-ending story. Cell Tissue Res. 2021; 383(1):409-427. PMC: 7878404. DOI: 10.1007/s00441-020-03349-9. View

15.

Takami S . Recent progress in the neurobiology of the vomeronasal organ. Microsc Res Tech. 2002; 58(3):228-50. DOI: 10.1002/jemt.10094. View

16.

Ibrahim D, Taniguchi K, Yamamoto Y, Taniguchi K, Nakamuta N . Immunohistochemical and lectin histochemical studies on the developing olfactory organs of fetal camel. Microsc Res Tech. 2015; 78(7):613-9. DOI: 10.1002/jemt.22517. View

17.

Yang W, Choi Y, Park C, Lee K, Ahn M, Kang W . Histological and lectin histochemical studies in the vomeronasal organ of the Korean black goat, Capra hircus coreanae. Acta Histochem. 2021; 123(2):151684. DOI: 10.1016/j.acthis.2021.151684. View

18.

Lin W, Ogura T, Margolskee R, Finger T, Restrepo D . TRPM5-expressing solitary chemosensory cells respond to odorous irritants. J Neurophysiol. 2007; 99(3):1451-60. DOI: 10.1152/jn.01195.2007. View

19.

Ibrahim D, Abdel-Maksoud F, Taniguchi K, Yamamoto Y, Taniguchi K, Nakamuta N . Immunohistochemical studies for the neuronal elements in the vomeronasal organ of the one-humped camel. J Vet Med Sci. 2014; 77(2):241-5. PMC: 4363031. DOI: 10.1292/jvms.14-0424. View

20.

Karimi H, Mansoori Ale Hashem R, Ardalani G, Sadrkhanloo R, Hayatgheibi H . Structure of vomeronasal organ (Jacobson organ) in male Camelus Domesticus Var. dromedaris persica. Anat Histol Embryol. 2014; 43(6):423-8. DOI: 10.1111/ahe.12092. View