Evaluation of Canine adipose-derived Mesenchymal Stem Cells for Neurological Functional Recovery in a Rat Model of Traumatic Brain Injury

Overview

Journal BMC Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2024 Mar 19

PMID 38500105

Authors

Wenkang Jiang

Huina Luo

Mingming Zhao

Quanbao Fan

Cailing Ye

Xingying Li

Jing He

Jianyi Lai

Shi He

Wojun Chen

Weihang Xian

Shengfeng Chen

Zhisheng Chen

Dongsheng Li

Ruiai Chen

Bingyun Wang

Affiliations

Soon will be listed here.

Abstract

Background: Traumatic brain injury (TBI) is a common condition in veterinary medicine that is difficult to manage.Veterinary regenerative therapy based on adipose mesenchymal stem cells seem to be an effective strategy for the treatment of traumatic brain injury. In this study, we evaluated therapeutic efficacy of canine Adipose-derived mesenchymal stem cells (AD-MSCs)in a rat TBI model, in terms of improved nerve function and anti-neuroinflammation.

Results: Canine AD-MSCs promoted neural functional recovery, reduced neuronal apoptosis, and inhibited the activation of microglia and astrocytes in TBI rats. According to the results in vivo, we further investigated the regulatory mechanism of AD-MSCs on activated microglia by co-culture in vitro. Finally, we found that canine AD-MSCs promoted their polarization to the M2 phenotype, and inhibited their polarization to the M1 phenotype. What's more, AD-MSCs could reduce the migration, proliferation and Inflammatory cytokines of activated microglia, which is able to inhibit inflammation in the central system.

Conclusions: Collectively, the present study demonstrates that transplantation of canine AD-MSCs can promote functional recovery in TBI rats via inhibition of neuronal apoptosis, glial cell activation and central system inflammation, thus providing a theoretical basis for canine AD-MSCs therapy for TBI in veterinary clinic.

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