KIF5B-mediated Internalization of FMDV Promotes Virus Infection

Overview

Journal Virol Sin

Specialty Microbiology

Date 2024 Mar 18

PMID 38499154

Authors

Wei Zhang

Fan Yang

Yang Yang

Weijun Cao

Wenhua Shao

Jiali Wang

Mengyao Huang

Zhitong Chen

Xiaoyi Zhao

Weiwei Li

Zixiang Zhu

Haixue Zheng

Affiliations

Soon will be listed here.

Abstract

Foot-and-mouth disease (FMD) is a highly contagious and economically important disease, which is caused by the FMD virus (FMDV). Although the cell receptor for FMDV has been identified, the specific mechanism of FMDV internalization after infection remains unknown. In this study, we found that kinesin family member 5B (KIF5B) plays a vital role during FMDV internalization. Moreover, we confirmed the interaction between KIF5B and FMDV structural protein VP1 by co-immunoprecipitation (Co-IP) and co-localization in FMDV-infected cells. In particular, the stalk [amino acids (aa) 413-678] domain of KIF5B was indispensable for KIF5B-VP1 interaction. Moreover, overexpression of KIF5B dramatically enhanced FMDV replication; consistently, knockdown or knockout of KIF5B suppressed FMDV replication. Furthermore, we also demonstrated that KIF5B promotes the internalization of FMDV via regulating clathrin uncoating. KIF5B also promotes the transmission of viral particles to early and late endosomes during the early stages of infection. In conclusion, our results demonstrate that KIF5B promotes the internalization of FMDV via regulating clathrin uncoating and intracellular transport. This study may provide a new therapeutic target for developing FMDV antiviral drugs.

Citing Articles

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PMID: 39507532 PMC: 11538601. DOI: 10.3389/fimmu.2024.1465374.

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