Construction of Recombinant Fluorescent LSDV for High-throughput Screening of Antiviral Drugs

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2024 Mar 17

PMID 38493160

Authors

Jingyu Wang

Jinzhao Ji

Yongcheng Zhong

Wenxin Meng

Shaobin Wan

Xiaoqing Ding

Zihan Chen

Weiyong Wu

Kun Jia

Shoujun Li

Affiliations

Soon will be listed here.

Abstract

Lumpy skin disease virus (LSDV) infection is a major socio-economic issue that seriously threatens the global cattle-farming industry. Here, a recombinant virus LSDV-ΔTK/EGFP, expressing enhanced green fluorescent protein (EGFP), was constructed with a homologous recombination system and applied to the high-throughput screening of antiviral drugs. LSDV-ΔTK/EGFP replicates in various kidney cell lines, consistent with wild-type LSDV. The cytopathic effect, viral particle morphology, and growth performance of LSDV-ΔTK/EGFP are consistent with those of wild-type LSDV. High-throughput screening allowed to identify several molecules that inhibit LSDV-ΔTK/EGFP replication. The strong inhibitory effect of theaflavin on LSDV was identified when 100 antiviral drugs were screened in vitro. An infection time analysis showed that theaflavin plays a role in the entry of LSDV into cells and in subsequent viral replication stages. The development of this recombinant virus will contribute to the development of LSDV-directed antiviral drugs and the study of viral replication and mechanisms of action.

Citing Articles

Potential Inhibitors of Lumpy Skin Disease's Viral Protein (DNA Polymerase): A Combination of Bioinformatics Approaches.

Zia S, Sumon M, Ashik M, Basar A, Lim S, Oh Y Animals (Basel). 2024; 14(9).

PMID: 38731287 PMC: 11083254. DOI: 10.3390/ani14091283.

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