Quantitative Mass Spectrometry Imaging (qMSI): A Tutorial

Overview

Journal J Mass Spectrom

Publisher Wiley

Date 2024 Mar 15

PMID 38488849

Authors

Russell R Kibbe

David C Muddiman

Affiliations

Soon will be listed here.

Abstract

Mass spectrometry imaging (MSI) is an analytical technique that enables the simultaneous detection of hundreds to thousands of chemical species while retaining their spatial information; usually, MSI is applied to biological tissues. Combining these elements can create ion images, which allows for the identification and localization of multiple chemical species within the sample. Being able to produce molecular images of biological tissues has already impacted the study of health and disease; however, the next logical step is being able to combine MSI with quantitative mass spectrometry methods to both quantify and determine the localization of disease progression or drug action. In this tutorial, we will detail the main factors to consider when designing a qMSI experiment and highlight the methods that have been developed to overcome these added complexities, specifically for those newer to the field of MSI.

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References

Lanekoff I, Thomas M, Carson J, Smith J, Timchalk C, Laskin J . Imaging nicotine in rat brain tissue by use of nanospray desorption electrospray ionization mass spectrometry. Anal Chem. 2012; 85(2):882-9. DOI: 10.1021/ac302308p. View

Zelows M, Cady C, Dharanipragada N, Mead A, Kipp Z, Bates E . Loss of carnitine palmitoyltransferase 1a reduces docosahexaenoic acid-containing phospholipids and drives sexually dimorphic liver disease in mice. Mol Metab. 2023; 78:101815. PMC: 10568566. DOI: 10.1016/j.molmet.2023.101815. View

Groseclose M, Castellino S . A mimetic tissue model for the quantification of drug distributions by MALDI imaging mass spectrometry. Anal Chem. 2013; 85(21):10099-106. DOI: 10.1021/ac400892z. View

Bergman H, Lundin E, Andersson M, Lanekoff I . Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization. Analyst. 2016; 141(12):3686-95. DOI: 10.1039/c5an02620b. View

Aikawa H, Hayashi M, Ryu S, Yamashita M, Ohtsuka N, Nishidate M . Visualizing spatial distribution of alectinib in murine brain using quantitative mass spectrometry imaging. Sci Rep. 2016; 6:23749. PMC: 4812395. DOI: 10.1038/srep23749. View

Hamm G, Bonnel D, Legouffe R, Pamelard F, Delbos J, Bouzom F . Quantitative mass spectrometry imaging of propranolol and olanzapine using tissue extinction calculation as normalization factor. J Proteomics. 2012; 75(16):4952-4961. DOI: 10.1016/j.jprot.2012.07.035. View

Neilson K, Ali N, Muralidharan S, Mirzaei M, Mariani M, Assadourian G . Less label, more free: approaches in label-free quantitative mass spectrometry. Proteomics. 2011; 11(4):535-53. DOI: 10.1002/pmic.201000553. View

Kibbe R, Muddiman D . Achieving Sub-Parts-per-Million Mass Measurement Accuracy on an Orbitrap Mass Spectrometry Imaging Platform without Automatic Gain Control. J Am Soc Mass Spectrom. 2023; 34(6):1015-1023. DOI: 10.1021/jasms.3c00004. View

Tu A, Muddiman D . Systematic evaluation of repeatability of IR-MALDESI-MS and normalization strategies for correcting the analytical variation and improving image quality. Anal Bioanal Chem. 2019; 411(22):5729-5743. PMC: 6706294. DOI: 10.1007/s00216-019-01953-5. View

10.

Dreisewerd K . Recent methodological advances in MALDI mass spectrometry. Anal Bioanal Chem. 2014; 406(9-10):2261-78. DOI: 10.1007/s00216-014-7646-6. View

11.

Barry J, Groseclose M, Castellino S . Quantification and assessment of detection capability in imaging mass spectrometry using a revised mimetic tissue model. Bioanalysis. 2019; 11(11):1099-1116. DOI: 10.4155/bio-2019-0035. View

12.

Mellinger A, Kibbe R, Rabbani Z, Meritet D, Muddiman D, Gamcsik M . Mapping glycine uptake and its metabolic conversion to glutathione in mouse mammary tumors using functional mass spectrometry imaging. Free Radic Biol Med. 2022; 193(Pt 2):677-684. PMC: 9737053. DOI: 10.1016/j.freeradbiomed.2022.11.010. View

13.

Deininger S, Cornett D, Paape R, Becker M, Pineau C, Rauser S . Normalization in MALDI-TOF imaging datasets of proteins: practical considerations. Anal Bioanal Chem. 2011; 401(1):167-81. PMC: 3124646. DOI: 10.1007/s00216-011-4929-z. View

14.

Angelini R, Yutuc E, Wyatt M, Newton J, Yusuf F, Griffiths L . Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging. Anal Chem. 2021; 93(11):4932-4943. PMC: 7992047. DOI: 10.1021/acs.analchem.0c05399. View

15.

Lietz C, Gemperline E, Li L . Qualitative and quantitative mass spectrometry imaging of drugs and metabolites. Adv Drug Deliv Rev. 2013; 65(8):1074-85. PMC: 3737419. DOI: 10.1016/j.addr.2013.04.009. View

16.

Nazari M, Bokhart M, Loziuk P, Muddiman D . Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI). Analyst. 2018; 143(3):654-661. PMC: 5788707. DOI: 10.1039/c7an01828b. View

17.

Wang Q, Ding S, Li Y, Royall J, Feng D, Lesnar P . The Allen Mouse Brain Common Coordinate Framework: A 3D Reference Atlas. Cell. 2020; 181(4):936-953.e20. PMC: 8152789. DOI: 10.1016/j.cell.2020.04.007. View

18.

Knochenmuss R, Zenobi R . MALDI ionization: the role of in-plume processes. Chem Rev. 2003; 103(2):441-52. DOI: 10.1021/cr0103773. View

19.

Lanekoff I, Stevens S, Stenzel-Poore M, Laskin J . Matrix effects in biological mass spectrometry imaging: identification and compensation. Analyst. 2014; 139(14):3528-32. PMC: 4078919. DOI: 10.1039/c4an00504j. View

20.

Taylor A, Dexter A, Bunch J . Exploring Ion Suppression in Mass Spectrometry Imaging of a Heterogeneous Tissue. Anal Chem. 2018; 90(9):5637-5645. DOI: 10.1021/acs.analchem.7b05005. View