Precisely Modulating the Chromatin Tracker Substituent Engineering: Reporting Pathological Oxidative Stress During Mitosis

Overview

Journal Chem Sci

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2024 Mar 15

PMID 38487223

Authors

JinSong Li

Yingyong Ni

Junjun Wang

Yicai Zhu

Aidong Wang

Xiaojiao Zhu

Xianshun Sun

Sen Wang

Dandan Li

Hongping Zhou

Affiliations

Soon will be listed here.

Abstract

An in-depth understanding of cancer-cell mitosis presents unprecedented advantages for solving metastasis and proliferation of tumors, which has aroused great interest in visualizing the behavior a luminescence tool. We developed a fluorescent molecule CBTZ-yne based on substituent engineering to acquire befitting lipophilicity and electrophilicity for anchoring lipid droplets and the nucleus, in which the low polarity environment and nucleic acids triggered a "weak-strong" fluorescence and "short-long" fluorescence-lifetime response. Meaningfully, CBTZ-yne visualized chromatin condensation, alignment, pull-push, and separation as well as lipid droplet dynamics, for the first time, precisely unveiling the asynchronous cellular mitosis processes affected by photo-generation reactive oxygen species according to the subtle change of fluorescence-lifetime. Our work suggested a new guideline for tracking the issue of the proliferation of malignant tumors in photodynamic therapy.

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