Comparative Analysis of Gut Microbiome in Pangasionodon Hypopthalmus and Labeo Catla During Health and Disease

Overview

Journal Int Microbiol

Publisher Springer Nature

Specialty Microbiology

Date 2024 Mar 14

PMID 38483744

Authors

R Bharathi Rathinam

Gayatri Tripathi

Basanta Kumar Das

Ratnesh Jain

Arpit Acharya

Affiliations

Soon will be listed here.

Abstract

The present study was conducted to study the composition of gut microbiome in the advanced fingerling and fingerling stage of striped pangasius catfish and catla during healthy and diseased conditions. Healthy pangasius and catla fishes were obtained from commercial farms and injected with the LD dose of A. hydrophila. The intestinal samples from the control and injected group were collected and pooled for 16 s metagenomic analysis. Community analysis was performed by targeting the 16 s rRNA gene to explore and compare the gut microbiota composition of these fishes. The operational taxonomic units (OTUs) consisted of four major phyla: Bacteroidia, Proteobacteria, Firmicutes, and Actinobacteria. Alpha and beta diversity indices were carried out to understand the diversity of microbes within and between a sample. While comparing the advanced fingerling and fingerling stage gut microbiome of Pangasius catfish, the dominance of Proteobacteria was found in fingerlings, whereas Firmicutes and Bacteroides were found in advanced fingerlings. In catla, Proteobacteria and Bacteroides were predominant. Taxonomic abundance of the microbiota in control and diseased Pangasius and catla fishes at phylum, class, order, family, genus, and species levels were also depicted. The present study is the first of its kind, and it will help to identify the diversity of novel potential bacterial species involved in disease protection of fishes. It can lead to the development of sustainable prophylactic measures against (re-)emerging bacterial diseases in aquaculture.

Citing Articles

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PMID: 38915412 PMC: 11194362. DOI: 10.3389/fimmu.2024.1411544.

References

Bass D, Stentiford G, Wang H, Koskella B, Tyler C . The Pathobiome in Animal and Plant Diseases. Trends Ecol Evol. 2019; 34(11):996-1008. PMC: 7479508. DOI: 10.1016/j.tree.2019.07.012. View

Burgos F, Ray C, Arias C . Bacterial diversity and community structure of the intestinal microbiome of Channel Catfish (Ictalurus punctatus) during ontogenesis. Syst Appl Microbiol. 2018; 41(5):494-505. DOI: 10.1016/j.syapm.2018.04.006. View

Colin Y, Molbert N, Berthe T, Agostini S, Alliot F, Decenciere B . Dysbiosis of fish gut microbiota is associated with helminths parasitism rather than exposure to PAHs at environmentally relevant concentrations. Sci Rep. 2022; 12(1):11084. PMC: 9246949. DOI: 10.1038/s41598-022-15010-2. View

Das S, Ward L, Burke C . Prospects of using marine actinobacteria as probiotics in aquaculture. Appl Microbiol Biotechnol. 2008; 81(3):419-29. DOI: 10.1007/s00253-008-1731-8. View

Dehler C, Secombes C, Martin S . Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr ( L.). Aquaculture. 2017; 467:149-157. PMC: 5142738. DOI: 10.1016/j.aquaculture.2016.07.017. View

Durack J, Lynch S . The gut microbiome: Relationships with disease and opportunities for therapy. J Exp Med. 2018; 216(1):20-40. PMC: 6314516. DOI: 10.1084/jem.20180448. View

Freitas S, Hatosy S, Fuhrman J, Huse S, Mark Welch D, Sogin M . Global distribution and diversity of marine Verrucomicrobia. ISME J. 2012; 6(8):1499-505. PMC: 3400412. DOI: 10.1038/ismej.2012.3. View

Gajardo K, Rodiles A, Kortner T, Krogdahl A, Bakke A, Merrifield D . A high-resolution map of the gut microbiota in Atlantic salmon (Salmo salar): A basis for comparative gut microbial research. Sci Rep. 2016; 6:30893. PMC: 4971465. DOI: 10.1038/srep30893. View

Gupta S, Feckaninova A, Lokesh J, Koscova J, Sorensen M, Fernandes J . Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics. Front Microbiol. 2019; 9:3247. PMC: 6343587. DOI: 10.3389/fmicb.2018.03247. View

10.

Hakim J, Koo H, Dennis L, Kumar R, Ptacek T, Morrow C . An abundance of Epsilonproteobacteria revealed in the gut microbiome of the laboratory cultured sea urchin, Lytechinus variegatus. Front Microbiol. 2015; 6:1047. PMC: 4602345. DOI: 10.3389/fmicb.2015.01047. View

11.

Ingerslev H, Strube M, Jorgensen L, Dalsgaard I, Boye M, Madsen L . Diet type dictates the gut microbiota and the immune response against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol. 2014; 40(2):624-33. DOI: 10.1016/j.fsi.2014.08.021. View

12.

Iorizzo M, Albanese G, Testa B, Ianiro M, Letizia F, Succi M . Presence of Lactic Acid Bacteria in the Intestinal Tract of the Mediterranean Trout () in Its Natural Environment. Life (Basel). 2021; 11(7). PMC: 8306010. DOI: 10.3390/life11070667. View

13.

Jami M, Ghanbari M, Kneifel W, Domig K . Phylogenetic diversity and biological activity of culturable Actinobacteria isolated from freshwater fish gut microbiota. Microbiol Res. 2015; 175:6-15. DOI: 10.1016/j.micres.2015.01.009. View

14.

Jian H, Liu Y, Wang X, Dong X, Zou X . as a Next-Generation Probiotic in Modulating Human Metabolic Homeostasis and Disease Progression: A Role Mediated by Gut-Liver-Brain Axes?. Int J Mol Sci. 2023; 24(4). PMC: 9959343. DOI: 10.3390/ijms24043900. View

15.

Kashinskaya E, Belkova N, Izvekova G, Simonov E, Andree K, Glupov V . A comparative study on microbiota from the intestine of Prussian carp (Carassius gibelio) and their aquatic environmental compartments, using different molecular methods. J Appl Microbiol. 2015; 119(4):948-61. DOI: 10.1111/jam.12904. View

16.

Kim P, Shin N, Lee J, Kim M, Whon T, Hyun D . Host habitat is the major determinant of the gut microbiome of fish. Microbiome. 2021; 9(1):166. PMC: 8325807. DOI: 10.1186/s40168-021-01113-x. View

17.

Larsen A, Mohammed H, Arias C . Characterization of the gut microbiota of three commercially valuable warmwater fish species. J Appl Microbiol. 2014; 116(6):1396-404. DOI: 10.1111/jam.12475. View

18.

Li H, Zhou Y, Ling H, Luo L, Qi D, Feng L . The effect of dietary supplementation with Clostridium butyricum on the growth performance, immunity, intestinal microbiota and disease resistance of tilapia (Oreochromis niloticus). PLoS One. 2019; 14(12):e0223428. PMC: 6901227. DOI: 10.1371/journal.pone.0223428. View

19.

Liu X, Wu H, Chang X, Tang Y, Liu Q, Zhang Y . Notable mucosal immune responses induced in the intestine of zebrafish (Danio rerio) bath-vaccinated with a live attenuated Vibrio anguillarum vaccine. Fish Shellfish Immunol. 2014; 40(1):99-108. DOI: 10.1016/j.fsi.2014.06.030. View

20.

Llewellyn M, McGinnity P, Dionne M, Letourneau J, Thonier F, Carvalho G . The biogeography of the atlantic salmon (Salmo salar) gut microbiome. ISME J. 2015; 10(5):1280-4. PMC: 5029221. DOI: 10.1038/ismej.2015.189. View