Transferrin in Isolated Cells from Rat Duodenum and Jejunum

Overview

Journal Blut

Specialty Hematology

Date 1985 Jul 1

PMID 3848337

Citations 5

Authors

K Osterloh

K Schumann

C Ehtechami

W Forth

Affiliations

Soon will be listed here.

Abstract

Mucosal transferrin was determined as transferrin-like immunoreactivity (TLIR) by means of a 2-site immunoradiometric assay (IRMA). Scraped-off mucosal tissue as well as isolated mucosal cells from the duodenum and jejunum of normal and iron-deficient rats before and after a washing procedure were examined. In iron-deficient rats there was about twice as much TLIR in scraped-off mucosal tissue as in the untreated animals. In the duodenum and jejunum of normal and iron-deficient rats, TLIR contents of the isolated cells in the magnitude of 320-510 ng/mg dry weight were found. Washing isolated cells three times in ice-cold Hank's solution resulted in a nearly tenfold decrease of TLIR content in all groups. In contrast the cells' RNA content remained unchanged.

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Intestinal iron absorption and mucosal transferrin in rats subjected to hypoxia.

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References

Forth W, Andres H . [Method for induction of iron deficiency in rats]. Arzneimittelforschung. 1969; 19(3):363-4. View

Bhargava M, GABBE E . Chronic experimental iron losses in rats not leading to overt iron deficiency: a model for the regulation of the whole-body iron balance in an iron-replete condition. J Nutr. 1984; 114(6):1060-9. DOI: 10.1093/jn/114.6.1060. View

Hatcher D, Goldstein G . Improved methods for determination of RNA and DNA. Anal Biochem. 1969; 31(1):42-50. DOI: 10.1016/0003-2697(69)90239-5. View

Bates G, Wernicke J . The kinetics and mechanism of iron(3) exchange between chelates and transferrin. IV. The reaction of transferrin with iron(3) nitrilotriacetate. J Biol Chem. 1971; 246(11):3679-85. View

Mason D, Taylor C . Distribution of transferrin, ferritin, and lactoferrin in human tissues. J Clin Pathol. 1978; 31(4):316-27. PMC: 1145269. DOI: 10.1136/jcp.31.4.316. View

Isobe K, Isobe Y . Localization of transferrin in rat duodenal mucosa by immunoperoxidase technique. Nihon Ketsueki Gakkai Zasshi. 1983; 46(4):797-807. View

Mitjavila M, Mitjavila S, DERACHE R . [Measurements of metabolism and lysis during the incubation of epithelial cells isolated from small intestine of the rat in presence of various surface-active agents (author's transl)]. Toxicology. 1973; 1(3):237-48. DOI: 10.1016/0300-483x(73)90011-5. View

Heytler P, Prichard W . A new class of uncoupling agents--carbonyl cyanide phenylhydrazones. Biochem Biophys Res Commun. 1962; 7:272-5. DOI: 10.1016/0006-291x(62)90189-4. View

Weiser M . Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation. J Biol Chem. 1973; 248(7):2536-41. View

10.

Osterloh K, Forth W . Determination of transferrin-like immunoreactivity in the mucosal homogenate of the duodenum, jejunum, and ileum of normal and iron deficient rats. Blut. 1981; 43(4):227-35. DOI: 10.1007/BF00320451. View

11.

Pfaff E, Schuler B, Krell H, Hoke H . Viability control and special properties of isolated rat hepatocytes. Arch Toxicol. 1980; 44(1-3):3-21. DOI: 10.1007/BF00303179. View

12.

Schreiber G, Dryburgh H, Millership A, Matsuda Y, Inglis A, Phillips J . The synthesis and secretion of rat transferrin. J Biol Chem. 1979; 254(23):12013-9. View

13.

Savin M, Cook J . Mucosal iron transport by rat intestine. Blood. 1980; 56(6):1029-35. View

14.

Vretblad P, Hjorth R . The use of wheat-germ lectin-Sepharose for the purification of human haemopexin. Biochem J. 1977; 167(3):759-64. PMC: 1183724. DOI: 10.1042/bj1670759. View