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CircAKT3 Alleviates Postoperative Cognitive Dysfunction by Stabilizing the Feedback Cycle of MiR-106a-5p/HDAC4/MEF2C Axis in Hippocampi of Aged Mice

Overview
Publisher Springer
Specialty Biology
Date 2024 Mar 13
PMID 38478029
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Abstract

Circular RNAs (circRNAs) have garnered significant attention in the field of neurodegenerative diseases including Alzheimer's diseases due to their covalently closed loop structure. However, the involvement of circRNAs in postoperative cognitive dysfunction (POCD) is still largely unexplored. To identify the genes differentially expressed between non-POCD (NPOCD) and POCD mice, we conducted the whole transcriptome sequencing initially in this study. According to the expression profiles, we observed that circAKT3 was associated with hippocampal neuronal apoptosis in POCD mice. Moreover, we found that circAKT3 overexpression reduced apoptosis of hippocampal neurons and alleviated POCD. Subsequently, through bioinformatics analysis, our data showed that circAKT3 overexpression in vitro and in vivo elevated the abundance of miR-106a-5p significantly, resulting in a decrease of HDAC4 protein and an increase of MEF2C protein. Additionally, this effect of circAKT3 was blocked by miR-106a-5p inhibitor. Interestingly, MEF2C could activate the transcription of miR-106a-5p promoter and form a positive feedback loop. Therefore, our findings revealed more potential modulation ways between circRNA-miRNA and miRNA-mRNA, providing different directions and targets for preclinical studies of POCD.

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References
1.
Quambusch L, Depta L, Landel I, Lubeck M, Kirschner T, Nabert J . Cellular model system to dissect the isoform-selectivity of Akt inhibitors. Nat Commun. 2021; 12(1):5297. PMC: 8421423. DOI: 10.1038/s41467-021-25512-8. View

2.
Sharma M, Dey C . Role of Akt isoforms in neuronal insulin signaling and resistance. Cell Mol Life Sci. 2021; 78(23):7873-7898. PMC: 11073101. DOI: 10.1007/s00018-021-03993-6. View

3.
Sun L, Dong R, Xu X, Yang X, Peng M . Activation of cannabinoid receptor type 2 attenuates surgery-induced cognitive impairment in mice through anti-inflammatory activity. J Neuroinflammation. 2017; 14(1):138. PMC: 5518095. DOI: 10.1186/s12974-017-0913-7. View

4.
Chen S, Zhou Y, Chen Y, Gu J . fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 2018; 34(17):i884-i890. PMC: 6129281. DOI: 10.1093/bioinformatics/bty560. View

5.
Shen W, Chen S, Gan Z, Zhang Y, Yue T, Chen M . AnimalTFDB 4.0: a comprehensive animal transcription factor database updated with variation and expression annotations. Nucleic Acids Res. 2022; 51(D1):D39-D45. PMC: 9825474. DOI: 10.1093/nar/gkac907. View