Eco-Friendly and Efficient Extraction of Polysaccharides from by Ultrasound-Assisted Deep Eutectic Solvent

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2024 Mar 13

PMID 38474454

Authors

Jiaojiao Xue

Jianqing Su

Xueyan Wang

Rui Zhang

Xiaoli Li

Ying Li

Yi Ding

Xiuling Chu

Affiliations

Soon will be listed here.

Abstract

A green extraction method was developed using deep eutectic solvent extraction for the polysaccharide from (). Among the eight types of DES prepared, the DES with a ratio of 1:4 L-malic acid to L-proline was found to be a suitable extraction solvent based on the extraction efficiency. The extraction parameters were optimized by Plackett-Burman and response surface methodology (RSM). The best extraction conditions were found for L-malic acid. Under the conditions of an L-malic acid/L-proline ratio of 1:4, ultrasonic power of 240 W, material-liquid ratio of 31.068 g/mL, water content of 32.364%, extraction time of 129.119 min, and extraction temperature of 60 °C, the extraction rate of polysaccharides was 35.452 ± 0.388 mg-g. This rate was higher than that of polysaccharides obtained by hot water extraction (13.652 ± 0.09 mg-g). The experimental results were best fitted by the quasi-secondary kinetic model when compared to two other kinetic models. Electron microscopic observations showed that DESs were more destructive to plant cells. The polysaccharide extracted from DESs had more monosaccharide components, a lower molecular weight, a higher antioxidant capacity, and superior anti-glycation activity compared to polysaccharides extracted from water (ASPS-PW). This study demonstrates the effectiveness of DESs in obtaining polysaccharides from .

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References

Han J, Liu L, Yu N, Chen J, Liu B, Yang D . Polysaccharides from Acanthopanax senticosus enhances intestinal integrity through inhibiting TLR4/NF-κB signaling pathways in lipopolysaccharide-challenged mice. Anim Sci J. 2015; 87(8):1011-8. DOI: 10.1111/asj.12528. View

Ullah S, Hussain S, Shaukat F, Hameed A, Yang W, Song Y . Antioxidant Potential and the Characterization of Roots. Biomed Res Int. 2020; 2019:7073456. PMC: 6948283. DOI: 10.1155/2019/7073456. View

Taweekayujan S, Somngam S, Pinnarat T . Optimization and kinetics modeling of phenolics extraction from coffee silverskin in deep eutectic solvent using ultrasound-assisted extraction. Heliyon. 2023; 9(7):e17942. PMC: 10336794. DOI: 10.1016/j.heliyon.2023.e17942. View

Fan Y, Ma X, Zhang J, Ma L, Gao Y, Zhang W . Ophiopogon polysaccharide liposome can enhance the non-specific and specific immune response in chickens. Carbohydr Polym. 2015; 119:219-27. DOI: 10.1016/j.carbpol.2014.11.048. View

Hammond O, Bowron D, Edler K . The Effect of Water upon Deep Eutectic Solvent Nanostructure: An Unusual Transition from Ionic Mixture to Aqueous Solution. Angew Chem Int Ed Engl. 2017; 56(33):9782-9785. PMC: 5596335. DOI: 10.1002/anie.201702486. View

Zhuang B, Dou L, Li P, Liu E . Deep eutectic solvents as green media for extraction of flavonoid glycosides and aglycones from Platycladi Cacumen. J Pharm Biomed Anal. 2016; 134:214-219. DOI: 10.1016/j.jpba.2016.11.049. View

Zhang Y, Zhang Y, Liu Z . Effects of supplementation on innate immunity and changes of related immune factors in healthy mice. Innate Immun. 2020; 27(6):461-469. PMC: 8504262. DOI: 10.1177/1753425920955200. View

Kim Y, Cho M, Kim D, Shin G, Lee J, Lee J . The antioxidant activity and their major antioxidant compounds from Acanthopanax senticosus and A. koreanum. Molecules. 2015; 20(7):13281-95. PMC: 6331968. DOI: 10.3390/molecules200713281. View

Yim S, Kim K, Kim I, Chun S, Oh T, Kim J . Inhibition of SARS-CoV-2 Virus Entry by the Crude Polysaccharides of Seaweeds and Abalone Viscera In Vitro. Mar Drugs. 2021; 19(4). PMC: 8071526. DOI: 10.3390/md19040219. View

10.

Tan H, Gan C . Polysaccharide with antioxidant, α-amylase inhibitory and ACE inhibitory activities from Momordica charantia. Int J Biol Macromol. 2016; 85:487-96. DOI: 10.1016/j.ijbiomac.2016.01.023. View

11.

Nishida M, Kondo M, Shimizu T, Saito T, Sato S, Hirayama M . Antihyperlipidemic effect of Acanthopanax senticosus (Rupr. et Maxim) Harms leaves in high-fat-diet fed mice. J Sci Food Agric. 2015; 96(11):3717-22. DOI: 10.1002/jsfa.7557. View

12.

Zhang P, Liu X, Liu H, Wang W, Liu X, Li X . Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication. Microb Pathog. 2017; 114:124-128. PMC: 7126552. DOI: 10.1016/j.micpath.2017.11.026. View

13.

Cvjetko Bubalo M, Curko N, Tomasevic M, Kovacevic Ganic K, Radojcic Redovnikovic I . Green extraction of grape skin phenolics by using deep eutectic solvents. Food Chem. 2016; 200:159-66. DOI: 10.1016/j.foodchem.2016.01.040. View

14.

Hwang E, Kim G, Song K, Lee H, Kim S . The enhancing effect of Acanthopanax sessiliflorus fruit extract on the antibacterial activity of porcine alveolar 3D4/31 macrophages via NF-κB1 and lipid metabolism regulation. Asian-Australas J Anim Sci. 2019; 32(11):1776-1788. PMC: 6817778. DOI: 10.5713/ajas.18.0874. View

15.

Li S, Gao A, Dong S, Chen Y, Sun S, Lei Z . Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta. Int J Biol Macromol. 2016; 96:26-34. DOI: 10.1016/j.ijbiomac.2016.12.007. View

16.

Kalhor P, Ghandi K . Deep Eutectic Solvents for Pretreatment, Extraction, and Catalysis of Biomass and Food Waste. Molecules. 2019; 24(22). PMC: 6891572. DOI: 10.3390/molecules24224012. View

17.

Li H, Qiang J, Song C, Xu P . Transcriptome profiling reveal Acanthopanax senticosus improves growth performance, immunity and antioxidant capacity by regulating lipid metabolism in GIFT (Oreochromis niloticus). Comp Biochem Physiol Part D Genomics Proteomics. 2020; 37:100784. DOI: 10.1016/j.cbd.2020.100784. View

18.

Sheldon R, Woodley J . Role of Biocatalysis in Sustainable Chemistry. Chem Rev. 2017; 118(2):801-838. DOI: 10.1021/acs.chemrev.7b00203. View

19.

Zhao Z, Xu X, Ye Q, Dong L . Ultrasound extraction optimization of Acanthopanax senticosus polysaccharides and its antioxidant activity. Int J Biol Macromol. 2013; 59:290-4. DOI: 10.1016/j.ijbiomac.2013.04.067. View

20.

Guo S, Wang J, He C, Wei H, Ma Y, Xiong H . Preparation and antioxidant activities of polysaccharides obtained from abalone viscera by combination of enzymolysis and multiple separation methods. J Food Sci. 2020; 85(12):4260-4270. DOI: 10.1111/1750-3841.15520. View