The Crucial Role of SlGSNOR in Regulating Postharvest Tomato Fruit Ripening

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Mar 13

PMID 38473974

Authors

Zesheng Liu

Dengjing Huang

Yandong Yao

Xuejuan Pan

Yanqin Zhang

Yi Huang

Zhiqi Ding

Chunlei Wang

Weibiao Liao

Affiliations

Soon will be listed here.

Abstract

-nitrosoglutathione reductase (GSNOR) is a well-known regulator in controlling protein -nitrosylation modification and nitric oxide (NO) homeostasis. Here, a GSNOR inhibitor N6022 and silencing were applied to investigate the roles of SlGSNOR in tomato fruit postharvest ripening. We found that the application of N6022 and -nitrosoglutathione (GSNO, a NO donor), and silencing delayed the transition of fruit skin color by improving total chlorophyll level by 88.57%, 44.78%, and 91.03%, respectively. Meanwhile, total carotenoid and lycopene contents were reduced by these treatments. Concurrently, the activity of chlorophyll biosynthesis enzymes and the expression of related genes were upregulated, and the transcript abundances of total carotenoid bioproduction genes were downregulated, by N6022 and GSNO treatments and silencing. In addition, fruit softening was postponed by N6022, GSNO, and silencing, through delaying the decrease of firmness and declining cell wall composition; structure-related enzyme activity; and gene expression levels. Furthermore, N6022, GSNO, and silencing enhanced the accumulation of titratable acid; ascorbic acid; total phenol; and total flavonoid, but repressed the content of soluble sugar and soluble protein accompanied with the expression pattern changes of nutrition-related genes. In addition, the endogenous NO contents were elevated by 197.55%; 404.59%; and 713.46%, and the endogenous SNOs contents were enhanced by 74.65%; 93.49%; and 94.85%; by N6022 and GSNO treatments and silencing, respectively. Altogether, these results indicate that SlGSNOR positively promotes tomato postharvest fruit ripening, which may be largely on account of its negative roles in the endogenous NO level.

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