Ciprofloxacin-, Cefazolin-, and Methicilin-Soaked Graphene Paper As an Antibacterial Medium Suppressing Cell Growth

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Mar 13

PMID 38473931

Authors

Barbara Nasilowska

Aneta Bombalska

Marta Kutwin

Agata Lange

Slawomir Jaworski

Kamila Narojczyk

Klaudia Olkowicz

Zdzislaw Bogdanowicz

Affiliations

Soon will be listed here.

Abstract

This paper presents the results of research on the impact of graphene paper on selected bacterial strains. Graphene oxide, from which graphene paper is made, has mainly bacteriostatic properties. Therefore, the main goal of this research was to determine the possibility of using graphene paper as a carrier of a medicinal substance. Studies of the degree of bacterial inhibition were performed on and strains. Graphene paper was analyzed not only in the state of delivery but also after the incorporation of the antibiotics ciprofloxacin, cefazolin, and methicillin into its structures. In addition, Fourier-Transform Infrared Spectroscopy, contact angle, and microscopic analysis of bacteria on the surface of the examined graphene paper samples were also performed. Studies have shown that graphene paper with built-in ciprofloxacin had a bactericidal effect on the strains of and . In contrast, methicillin, as well as cefazolin, deposited on graphene paper acted mainly locally. Studies have shown that graphene paper can be used as a carrier of selected medicinal substances.

Citing Articles

Effect of Doping Cement Mortar with Triclosan, Hypochlorous Acid, Silver Nanoparticles and Graphene Oxide on Its Mechanical and Biological Properties.

Paciejewski M, Lange A, Jaworski S, Kutwin M, Bombalska A, Siwinski J Materials (Basel). 2025; 17(24.

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Effect of Graphene Aerosol Doped with Hypochlorous Acid, Curcumin, and Silver Nanoparticles on Selected Structural and Biological Properties.

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References

Nanda S, Yi D, Kim K . Study of antibacterial mechanism of graphene oxide using Raman spectroscopy. Sci Rep. 2016; 6:28443. PMC: 4914938. DOI: 10.1038/srep28443. View

Wu X, Tan S, Xing Y, Pu Q, Wu M, Zhao J . Graphene oxide as an efficient antimicrobial nanomaterial for eradicating multi-drug resistant bacteria in vitro and in vivo. Colloids Surf B Biointerfaces. 2017; 157:1-9. DOI: 10.1016/j.colsurfb.2017.05.024. View

Kumar P, Huo P, Zhang R, Liu B . Antibacterial Properties of Graphene-Based Nanomaterials. Nanomaterials (Basel). 2019; 9(5). PMC: 6567318. DOI: 10.3390/nano9050737. View

Thebo K, Qian X, Zhang Q, Chen L, Cheng H, Ren W . Highly stable graphene-oxide-based membranes with superior permeability. Nat Commun. 2018; 9(1):1486. PMC: 5902455. DOI: 10.1038/s41467-018-03919-0. View

Yu C, Chen G, Xia M, Xie Y, Chi Y, He Z . Understanding the sheet size-antibacterial activity relationship of graphene oxide and the nano-bio interaction-based physical mechanisms. Colloids Surf B Biointerfaces. 2020; 191:111009. DOI: 10.1016/j.colsurfb.2020.111009. View

Ye J, Wang Y, Li Z, Yang D, Li C, Yan Y . 2D confinement freestanding graphene oxide composite membranes with enriched oxygen vacancies for enhanced organic contaminants removal via peroxymonosulfate activation. J Hazard Mater. 2021; 417:126028. DOI: 10.1016/j.jhazmat.2021.126028. View

Ng I, Shamsi S . Graphene Oxide (GO): A Promising Nanomaterial against Infectious Diseases Caused by Multidrug-Resistant Bacteria. Int J Mol Sci. 2022; 23(16). PMC: 9408893. DOI: 10.3390/ijms23169096. View

Nasilowska B, Bogdanowicz Z, Sarzynski A, Skrzeczanowski W, Djas M, Bartosewicz B . The Influence of Laser Ablation Parameters on the Holes Structure of Laser Manufactured Graphene Paper Microsieves. Materials (Basel). 2020; 13(7). PMC: 7177287. DOI: 10.3390/ma13071568. View

Aunkor M, Raihan T, Prodhan S, Metselaar H, Malik S, Azad A . Antibacterial activity of graphene oxide nanosheet against multidrug resistant superbugs isolated from infected patients. R Soc Open Sci. 2020; 7(7):200640. PMC: 7428267. DOI: 10.1098/rsos.200640. View

10.

Matulewicz K, Kazmierski L, Wisniewski M, Roszkowski S, Roszkowski K, Kowalczyk O . Ciprofloxacin and Graphene Oxide Combination-New Face of a Known Drug. Materials (Basel). 2020; 13(19). PMC: 7579301. DOI: 10.3390/ma13194224. View

11.

Karahan H, Wei L, Goh K, Liu Z, Birer O, Dehghani F . Bacterial physiology is a key modulator of the antibacterial activity of graphene oxide. Nanoscale. 2016; 8(39):17181-17189. DOI: 10.1039/c6nr05745d. View

12.

Lu X, Feng X, Werber J, Chu C, Zucker I, Kim J . Enhanced antibacterial activity through the controlled alignment of graphene oxide nanosheets. Proc Natl Acad Sci U S A. 2017; 114(46):E9793-E9801. PMC: 5699062. DOI: 10.1073/pnas.1710996114. View

13.

Ruiz O, Fernando K, Wang B, Brown N, Luo P, McNamara N . Graphene oxide: a nonspecific enhancer of cellular growth. ACS Nano. 2011; 5(10):8100-7. DOI: 10.1021/nn202699t. View

14.

Liu S, Hu M, Zeng T, Wu R, Jiang R, Wei J . Lateral dimension-dependent antibacterial activity of graphene oxide sheets. Langmuir. 2012; 28(33):12364-72. DOI: 10.1021/la3023908. View

15.

Gurunathan S, Han J, Abdal Dayem A, Eppakayala V, Kim J . Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa. Int J Nanomedicine. 2012; 7:5901-14. PMC: 3514835. DOI: 10.2147/IJN.S37397. View

16.

Nasilowska B, Bogdanowicz Z, Kasprzycka W, Bombalska A, Mierczyk Z . Studies on the Effect of Graphene Oxide Deposited on Gold and Nickel Microsieves on Prostate Cancer Cells DU 145. Int J Mol Sci. 2022; 23(12). PMC: 9224325. DOI: 10.3390/ijms23126567. View

17.

Zou F, Zhou H, Jeong D, Kwon J, Eom S, Park T . Wrinkled Surface-Mediated Antibacterial Activity of Graphene Oxide Nanosheets. ACS Appl Mater Interfaces. 2016; 9(2):1343-1351. DOI: 10.1021/acsami.6b15085. View

18.

Beaudoin T, Yau Y, Stapleton P, Gong Y, Wang P, Guttman D . interaction with biofilm enhances tobramycin resistance. NPJ Biofilms Microbiomes. 2017; 3:25. PMC: 5648753. DOI: 10.1038/s41522-017-0035-0. View

19.

Papaioannidou P, Nitsas V, Mirtsou-Fidani V . Hydrolysis of cefazolin by enzymes produced by Pseudomonas aeruginosa after exposure to ceftazidime in vitro. Vojnosanit Pregl. 2009; 66(10):785-90. DOI: 10.2298/vsp0910785p. View

20.

Ravikumar V, Mijakovic I, Pandit S . Antimicrobial Activity of Graphene Oxide Contributes to Alteration of Key Stress-Related and Membrane Bound Proteins. Int J Nanomedicine. 2023; 17:6707-6721. PMC: 9805717. DOI: 10.2147/IJN.S387590. View